Literature DB >> 27455460

Structural basis of Cas3 inhibition by the bacteriophage protein AcrF3.

Xiaofei Wang1, Deqiang Yao2, Jin-Gen Xu1, A-Rong Li1, Jianpo Xu1, Panhan Fu1, Yan Zhou1, Yongqun Zhu1.   

Abstract

Bacteriophages express proteins that inactivate the CRISPR-Cas bacterial immune system. Here we report the crystal structure of the anti-CRISPR protein AcrF3 in complex with Pseudomonas aeruginosa Cas3 (PaCas3). AcrF3 forms a homodimer that locks PaCas3 in an ADP-bound form, blocks the entrance of the DNA-binding tunnel in the helicase domain, and masks the linker region and C-terminal domain of PaCas3, thereby preventing recruitment by Cascade and inhibiting the type I-F CRISPR-Cas system.

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Year:  2016        PMID： 27455460     DOI： 10.1038/nsmb.3269

Source DB:  PubMed          Journal:  Nat Struct Mol Biol        ISSN： 1545-9985            Impact factor:   15.369


  17 in total

1.  Refinement of macromolecular structures by the maximum-likelihood method.

Authors:  G N Murshudov; A A Vagin; E J Dodson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1997-05-01

Review 2.  RNA-guided genetic silencing systems in bacteria and archaea.

Authors:  Blake Wiedenheft; Samuel H Sternberg; Jennifer A Doudna
Journal:  Nature       Date:  2012-02-15       Impact factor: 49.962

3.  Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems.

Authors:  Jiuyu Wang; Jiazhi Li; Hongtu Zhao; Gang Sheng; Min Wang; Maolu Yin; Yanli Wang
Journal:  Cell       Date:  2015-10-17       Impact factor: 41.582

4.  Processing of X-ray diffraction data collected in oscillation mode.

Authors:  Z Otwinowski; W Minor
Journal:  Methods Enzymol       Date:  1997       Impact factor: 1.600

5.  CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference.

Authors:  Megan L Hochstrasser; David W Taylor; Prashant Bhat; Chantal K Guegler; Samuel H Sternberg; Eva Nogales; Jennifer A Doudna
Journal:  Proc Natl Acad Sci U S A       Date:  2014-04-18       Impact factor: 11.205

6.  Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli.

Authors:  Robert P Hayes; Yibei Xiao; Fran Ding; Paul B G van Erp; Kanagalaghatta Rajashankar; Scott Bailey; Blake Wiedenheft; Ailong Ke
Journal:  Nature       Date:  2016-02-10       Impact factor: 49.962

Review 7.  An updated evolutionary classification of CRISPR-Cas systems.

Authors:  Kira S Makarova; Yuri I Wolf; Omer S Alkhnbashi; Fabrizio Costa; Shiraz A Shah; Sita J Saunders; Rodolphe Barrangou; Stan J J Brouns; Emmanuelle Charpentier; Daniel H Haft; Philippe Horvath; Sylvain Moineau; Francisco J M Mojica; Rebecca M Terns; Michael P Terns; Malcolm F White; Alexander F Yakunin; Roger A Garrett; John van der Oost; Rolf Backofen; Eugene V Koonin
Journal:  Nat Rev Microbiol       Date:  2015-09-28       Impact factor: 60.633

8.  In vitro reconstitution of an Escherichia coli RNA-guided immune system reveals unidirectional, ATP-dependent degradation of DNA target.

Authors:  Sabin Mulepati; Scott Bailey
Journal:  J Biol Chem       Date:  2013-06-11       Impact factor: 5.157

9.  Foreign DNA acquisition by the I-F CRISPR-Cas system requires all components of the interference machinery.

Authors:  Daria Vorontsova; Kirill A Datsenko; Sofia Medvedeva; Joseph Bondy-Denomy; Ekaterina E Savitskaya; Ksenia Pougach; Maria Logacheva; Blake Wiedenheft; Alan R Davidson; Konstantin Severinov; Ekaterina Semenova
Journal:  Nucleic Acids Res       Date:  2015-11-19       Impact factor: 16.971

10.  Foreign DNA capture during CRISPR-Cas adaptive immunity.

Authors:  James K Nuñez; Lucas B Harrington; Philip J Kranzusch; Alan N Engelman; Jennifer A Doudna
Journal:  Nature       Date:  2015-10-21       Impact factor: 49.962
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  52 in total

1.  Inhibition of CRISPR-Cas9 with Bacteriophage Proteins.

Authors:  Benjamin J Rauch; Melanie R Silvis; Judd F Hultquist; Christopher S Waters; Michael J McGregor; Nevan J Krogan; Joseph Bondy-Denomy
Journal:  Cell       Date:  2016-12-29       Impact factor: 41.582

2.  Structural insight into multistage inhibition of CRISPR-Cas12a by AcrVA4.

Authors:  Ruchao Peng; Zhiteng Li; Ying Xu; Shaoshuai He; Qi Peng; Lian-Ao Wu; Ying Wu; Jianxun Qi; Peiyi Wang; Yi Shi; George F Gao
Journal:  Proc Natl Acad Sci U S A       Date:  2019-08-29       Impact factor: 11.205

Review 3.  Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins.

Authors:  Ning Jia; Dinshaw J Patel
Journal:  Nat Rev Mol Cell Biol       Date:  2021-06-04       Impact factor: 94.444

4.  Cas1 and the Csy complex are opposing regulators of Cas2/3 nuclease activity.

Authors:  MaryClare F Rollins; Saikat Chowdhury; Joshua Carter; Sarah M Golden; Royce A Wilkinson; Joseph Bondy-Denomy; Gabriel C Lander; Blake Wiedenheft
Journal:  Proc Natl Acad Sci U S A       Date:  2017-04-24       Impact factor: 11.205

5.  Spacer capture and integration by a type I-F Cas1-Cas2-3 CRISPR adaptation complex.

Authors:  Robert D Fagerlund; Max E Wilkinson; Oleg Klykov; Arjan Barendregt; F Grant Pearce; Sebastian N Kieper; Howard W R Maxwell; Angela Capolupo; Albert J R Heck; Kurt L Krause; Mihnea Bostina; Richard A Scheltema; Raymond H J Staals; Peter C Fineran
Journal:  Proc Natl Acad Sci U S A       Date:  2017-06-13       Impact factor: 11.205

Review 6.  Inhibition of CRISPR-Cas systems by mobile genetic elements.

Authors:  Erik J Sontheimer; Alan R Davidson
Journal:  Curr Opin Microbiol       Date:  2017-06-29       Impact factor: 7.934

7.  Structure Reveals Mechanisms of Viral Suppressors that Intercept a CRISPR RNA-Guided Surveillance Complex.

Authors:  Saikat Chowdhury; Joshua Carter; MaryClare F Rollins; Sarah M Golden; Ryan N Jackson; Connor Hoffmann; Lyn'Al Nosaka; Joseph Bondy-Denomy; Karen L Maxwell; Alan R Davidson; Elizabeth R Fischer; Gabriel C Lander; Blake Wiedenheft
Journal:  Cell       Date:  2017-03-23       Impact factor: 41.582

8.  The domain architecture of the protozoan protein J-DNA-binding protein 1 suggests synergy between base J DNA binding and thymidine hydroxylase activity.

Authors:  Athanassios Adamopoulos; Tatjana Heidebrecht; Jeroen Roosendaal; Wouter G Touw; Isabelle Q Phan; Jos Beijnen; Anastassis Perrakis
Journal:  J Biol Chem       Date:  2019-07-10       Impact factor: 5.157

9.  A molecular arms race: new insights into anti-CRISPR mechanisms.

Authors:  John Mallon; Scott Bailey
Journal:  Nat Struct Mol Biol       Date:  2016-09-06       Impact factor: 15.369

10.  Bacteriophage Cooperation Suppresses CRISPR-Cas3 and Cas9 Immunity.

Authors:  Adair L Borges; Jenny Y Zhang; MaryClare F Rollins; Beatriz A Osuna; Blake Wiedenheft; Joseph Bondy-Denomy
Journal:  Cell       Date:  2018-07-19       Impact factor: 41.582
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