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Literature DB >> 27351550

High resolution observed in 800 MHz DNP spectra of extremely rigid type III secretion needles.

Pascal Fricke¹, Deni Mance², Veniamin Chevelkov¹, Karin Giller³, Stefan Becker³, Marc Baldus², Adam Lange^4,5.

Abstract

The cryogenic temperatures at which dynamic nuclear polarization (DNP) solid-state NMR experiments need to be carried out cause line-broadening, an effect that is especially detrimental for crowded protein spectra. By increasing the magnetic field strength from 600 to 800 MHz, the resolution of DNP spectra of type III secretion needles (T3SS) could be improved by 22 %, indicating that inhomogeneous broadening is not the dominant effect that limits the resolution of T3SS needles under DNP conditions. The outstanding spectral resolution of this system under DNP conditions can be attributed to its low overall flexibility.

Keywords: DNP; Dynamics; PRE; Proteins; Solid-State NMR; Type III Secretion System

Mesh：

Substances：

Year: 2016 PMID： 27351550 DOI： 10.1007/s10858-016-0044-y

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

39 in total

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4. Successive Stages of Amyloid-β Self-Assembly Characterized by Solid-State Nuclear Magnetic Resonance with Dynamic Nuclear Polarization.

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5. TOTAPOL: a biradical polarizing agent for dynamic nuclear polarization experiments in aqueous media.

Authors: Changsik Song; Kan-Nian Hu; Chan-Gyu Joo; Timothy M Swager; Robert G Griffin
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8. High-Field Dynamic Nuclear Polarization for Solid and Solution Biological NMR.

Authors: A B Barnes; G De Paëpe; P C A van der Wel; K-N Hu; C-G Joo; V S Bajaj; M L Mak-Jurkauskas; J R Sirigiri; J Herzfeld; R J Temkin; R G Griffin
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10. Atomic-resolution structure of cytoskeletal bactofilin by solid-state NMR.

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2. Dynamic Nuclear Polarization Magic-Angle Spinning Nuclear Magnetic Resonance Combined with Molecular Dynamics Simulations Permits Detection of Order and Disorder in Viral Assemblies.

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Journal: J Phys Chem B Date: 2019-06-11 Impact factor: 2.991

High resolution observed in 800 MHz DNP spectra of extremely rigid type III secretion needles.

1. Strategies for solid-state NMR investigations of supramolecular assemblies with large subunit sizes.

2. Structural heterogeneity in microcrystalline ubiquitin studied by solid-state NMR.

Review 3. Magic angle spinning NMR of proteins: high-frequency dynamic nuclear polarization and (1)H detection.

4. Successive Stages of Amyloid-β Self-Assembly Characterized by Solid-State Nuclear Magnetic Resonance with Dynamic Nuclear Polarization.

5. TOTAPOL: a biradical polarizing agent for dynamic nuclear polarization experiments in aqueous media.

6. Site-Resolved Backbone and Side-Chain Intermediate Dynamics in a Carbohydrate-Binding Module Protein Studied by Magic-Angle Spinning NMR Spectroscopy.

7. Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies.

8. High-Field Dynamic Nuclear Polarization for Solid and Solution Biological NMR.

9. Higher order amyloid fibril structure by MAS NMR and DNP spectroscopy.

10. Atomic-resolution structure of cytoskeletal bactofilin by solid-state NMR.

Review 1. New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR.

2. Dynamic Nuclear Polarization Magic-Angle Spinning Nuclear Magnetic Resonance Combined with Molecular Dynamics Simulations Permits Detection of Order and Disorder in Viral Assemblies.

3. Direct dynamic nuclear polarization of ¹⁵N and ¹³C spins at 14.1 T using a trityl radical and magic angle spinning.

4. Targetable Tetrazine-Based Dynamic Nuclear Polarization Agents for Biological Systems.

Review 5. High-sensitivity protein solid-state NMR spectroscopy.

6. ¹⁹F Dynamic Nuclear Polarization at Fast Magic Angle Spinning for NMR of HIV-1 Capsid Protein Assemblies.

7. Frequency-Swept Integrated and Stretched Solid Effect Dynamic Nuclear Polarization.

8. Peptide and Protein Dynamics and Low-Temperature/DNP Magic Angle Spinning NMR.

9. NMR Signal Quenching from Bound Biradical Affinity Reagents in DNP Samples.

10. Modular, triple-resonance, transmission line DNP MAS probe for 500 MHz/330 GHz.