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Literature DB >> 272635

Binding and release of eukaryotic initiation factor eIF-2 and GTP during protein synthesis initiation.

Abstract

The eukaryotic initiation factor eIF-2 forms a ternary complex with Met-tRNAf and GTP. This complex binds to the 40S ribosomal subunit in the absence of mRNA and mRNA binding factors. Highly purified eIF-2 from rabbit reticulocytes was labeled with 125I by using the Bolton-Hunter reagent or with [gamma-32P]ATP by using the heme-regulated translational inhibitor protein kinase. The labeled eIF-2 was bound, together with equimolar amounts of Met-tRNAf and GTP, to the 40S subunit. In the presence of mRNA, mRNA binding factors, and 60S ribosomal subunits (complete initiation assay), eIF-2 was released from the 40S initiation complex in the subunit joining reaction. GTP also was released in this step and probably was hydrolyzed in the reaction that is dependent upon eIF-5 and the 60S subunit. The function of phosphorylated eIF-2 in initiation of protein synthesis is discussed.

Entities: Chemical Gene Species

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Year: 1978 PMID： 272635 PMCID： PMC411214 DOI： 10.1073/pnas.75.1.204

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

21 in total

1. Partial reaction of peptide initiation inhibited by the reticulocyte hemin-controlled repressor.

Authors: P Pinphanichakarn; G Kramer; B Hardesty
Journal: Biochem Biophys Res Commun Date: 1976-12-06 Impact factor: 3.575

2. Regulation of protein synthesis in rabbit reticulocyte lysates: purification and initial characterization of the cyclic 3':5'-AMP independent protein kinase of the heme-regulated translational inhibitor.

Authors: R S Ranu; I M London
Journal: Proc Natl Acad Sci U S A Date: 1976-12 Impact factor: 11.205

3. On the mechanism of delayed inhibition of protein synthesis in heme-defecient rabbit reticulocyte lysates.

Authors: L Cherbas; I M London
Journal: Proc Natl Acad Sci U S A Date: 1976-10 Impact factor: 11.205

4. Protein synthesis initiation in eukaryotes. Characterization of ribosomal factors from mouse fibroblasts.

Authors: D H Levin; D Kyner; G Acs
Journal: J Biol Chem Date: 1973-09-25 Impact factor: 5.157

5. Initiation of eukaryotic protein synthesis: (Met-tRNA f -40S ribosome) initiation complex catalysed by purified initiation factors in the absence of mRNA.

Authors: M H Schreier; T Staehelin
Journal: Nat New Biol Date: 1973-03-14

6. Initiation of protein synthesis: evidence for messenger RNA-independent binding of methionyl-transfer RNA to the 40 S ribosomal subunit.

Authors: C Darnbrough; S Legon; T Hunt; R J Jackson
Journal: J Mol Biol Date: 1973-05-25 Impact factor: 5.469

7. Translational control in hemoglobin syntheskis.

Authors: M Rabinovitz; M L Freedman; J M Fisher; C R Maxwell
Journal: Cold Spring Harb Symp Quant Biol Date: 1969

8. Phosphorylation of initiation factor elF-2 and the control of reticulocyte protein synthesis.

Authors: P J Farrell; K Balkow; T Hunt; R J Jackson; H Trachsel
Journal: Cell Date: 1977-05 Impact factor: 41.582

9. Protein initiation in eukaryotes: formation and function of a ternary complex composed of a partially purified ribosomal factor, methionyl transfer RNA, and guanosine triphosphate.

Authors: D H Levin; D Kyner; G Acs
Journal: Proc Natl Acad Sci U S A Date: 1973-01 Impact factor: 11.205

10. Partial reaction of peptide initiation inhibited by phosphorylation of either initiation factor eIF-2 or 40S ribosomal proteins.

Authors: G Kramer; A B Henderson; P Pinphanichakarn; M H Wallis; B Hardesty
Journal: Proc Natl Acad Sci U S A Date: 1977-04 Impact factor: 11.205

24 in total

1. Control of eIF-2 phosphatase activity in rabbit reticulocyte lysate.

Authors: B Safer; R Jagus
Journal: Proc Natl Acad Sci U S A Date: 1979-03 Impact factor: 11.205

2. DDX6 recruits translational silenced human reticulocyte 15-lipoxygenase mRNA to RNP granules.

Authors: Isabel S Naarmann; Christiane Harnisch; Gerhard Müller-Newen; Henning Urlaub; Antje Ostareck-Lederer; Dirk H Ostareck
Journal: RNA Date: 2010-09-30 Impact factor: 4.942

3. The 90-kDa component of reticulocyte heme-regulated eIF-2 alpha (initiation factor 2 alpha-subunit) kinase is derived from the beta subunit of spectrin.

Authors: W Kudlicki; S Fullilove; G Kramer; B Hardesty
Journal: Proc Natl Acad Sci U S A Date: 1985-08 Impact factor: 11.205

4. Homologous segments in three subunits of the guanine nucleotide exchange factor eIF2B mediate translational regulation by phosphorylation of eIF2.

Authors: G D Pavitt; W Yang; A G Hinnebusch
Journal: Mol Cell Biol Date: 1997-03 Impact factor: 4.272

5. Regulation of protein synthesis in rabbit reticulocyte lysates: purification and characterization of heme-reversible translational inhibitor.

Authors: H Trachsel; R S Ranu; I M London
Journal: Proc Natl Acad Sci U S A Date: 1978-08 Impact factor: 11.205

6. Molecular cloning and characterization of cDNA encoding the alpha subunit of the rat protein synthesis initiation factor eIF-2B.

Authors: K M Flowers; S R Kimball; R C Feldhoff; A G Hinnebusch; L S Jefferson
Journal: Proc Natl Acad Sci U S A Date: 1995-05-09 Impact factor: 11.205

7. Effect of phosphorylation of the alpha-subunit of eukaryotic initiation factor 2 on the function of reversing factor in the initiation of protein synthesis.

Authors: R L Matts; D H Levin; I M London
Journal: Proc Natl Acad Sci U S A Date: 1983-05 Impact factor: 11.205