Literature DB >> 220606

Control of eIF-2 phosphatase activity in rabbit reticulocyte lysate.

B Safer, R Jagus.   

Abstract

A sensitive assay requiring picomole amounts of [32P]eIF-2 to measure eIF-2 phosphatase activity has been developed. Dephosphorylation of [32P]eIF-2 alpha (38,000-dalton subunit) is extremely rapid (t 1/2 = 20 sec) and occurs at the same rate in both hemin-supplemented and hemin-depleted lysates. In contrast, [32P]eIF-2 beta phosphate is stable under all conditions studied. At concentrations required to produce a transient inhibition of protein synthesis, GDP prevents dephosphorylation of half of the phosphate introduced on eIF-2 alpha by the hemin-controlled repressor. Equimolar GTP is without effect. The concept that the energy charge of the guanylate pool may regulate accessibility of a phosphorylated site on eIF-2 alpha to its phosphatase and the implication of this to the mechanism of hemin-regulated translational control are discussed.

Entities:  

Mesh：

Substances：

Year:  1979        PMID： 220606      PMCID： PMC383195          DOI： 10.1073/pnas.76.3.1094

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  29 in total

1.  On the mechanism of delayed inhibition of protein synthesis in heme-defecient rabbit reticulocyte lysates.

Authors:  L Cherbas; I M London
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205

2.  Factors involved in initiation of haemoglobin synthesis can be phosphorylated in vitro.

Authors:  J A Traugh; S M Tahara; S B Sharp; B Safer; W C Merrick
Journal:  Nature       Date:  1976-09-09       Impact factor: 49.962

3.  Regulation of protein synthesis in rabbit reticulocyte lysates: purification and characterization of heme-reversible translational inhibitor.

Authors:  H Trachsel; R S Ranu; I M London
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

Review 4.  The molecular mechanism of hemoglobin synthesis and its regulation in the reticulocyte.

Authors:  B Safer; W F Anderson
Journal:  CRC Crit Rev Biochem       Date:  1978

Review 5.  Post-transcriptional and translational controls of gene expression in eukaryotes.

Authors:  M Revel; Y Groner
Journal:  Annu Rev Biochem       Date:  1978       Impact factor: 23.643

6.  Analysis of initiation factor function in highly fractionated and unfractionated reticulocyte lysate systems.

Authors:  B Safer; R Jagus; W M Kemper
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

7.  Partial reaction of peptide initiation inhibited by phosphorylation of either initiation factor eIF-2 or 40S ribosomal proteins.

Authors:  G Kramer; A B Henderson; P Pinphanichakarn; M H Wallis; B Hardesty
Journal:  Proc Natl Acad Sci U S A       Date:  1977-04       Impact factor: 11.205

8.  Effect of hemin on site-specific phosphorylation of eukaryotic initiation factor 2.

Authors:  S M Tahara; J A Traugh; S B Sharp; T S Lundak; B Safer; W C Merrick
Journal:  Proc Natl Acad Sci U S A       Date:  1978-02       Impact factor: 11.205

9.  Regulation of protein synthesis in reticulocyte lysates: phosphorylation of methionyl-tRNAf binding factor by protein kinase activity of translational inhibitor isolated from hemedeficient lysates.

Authors:  D Levin; R S Ranu; V Ernst; I M London
Journal:  Proc Natl Acad Sci U S A       Date:  1976-09       Impact factor: 11.205

10.  Specificity of the protein kinase activity associated with the hemin-controlled repressor of rabbit reticulocyte.

Authors:  G Kramer; J M Cimadevilla; B Hardesty
Journal:  Proc Natl Acad Sci U S A       Date:  1976-09       Impact factor: 11.205
View more
  11 in total

Review 1.  Cell-free protein synthesis: applications come of age.

Authors:  Erik D Carlson; Rui Gan; C Eric Hodgman; Michael C Jewett
Journal:  Biotechnol Adv       Date:  2011-10-08       Impact factor: 14.227

2.  Protein synthesis in rabbit reticulocytes: characteristics of a postribosomal supernatant factor that reverses inhibition of protein synthesis in heme-deficient lysates and inhibition of ternary complex (Met-tRNAfMet.eIF-2.GTP) formation by heme-regulated inhibitor.

Authors:  R O Ralston; A Das; M Grace; H Das; N K Gupta
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205

Review 3.  Initiation of protein synthesis in mammalian cells.

Authors:  V M Pain
Journal:  Biochem J       Date:  1986-05-01       Impact factor: 3.857

4.  The control of protein synthesis by hemin in rabbit reticulocytes.

Authors:  M Gross
Journal:  Mol Cell Biochem       Date:  1980-05-28       Impact factor: 3.396

5.  Double-stranded RNA inhibits a phosphoprotein phosphatase present in interferon-treated cells.

Authors:  D A Epstein; P F Torrence; R M Friedman
Journal:  Proc Natl Acad Sci U S A       Date:  1980-01       Impact factor: 11.205

6.  Translational control by influenza virus: suppression of the kinase that phosphorylates the alpha subunit of initiation factor eIF-2 and selective translation of influenza viral mRNAs.

Authors:  M G Katze; B M Detjen; B Safer; R M Krug
Journal:  Mol Cell Biol       Date:  1986-05       Impact factor: 4.272

7.  Effects of skeletal muscle protein phosphatase inhibitor-2 on protein synthesis and protein phosphorylation in rabbit reticulocyte lysates.

Authors:  V Ernst; D H Levin; J G Foulkes; I M London
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

8.  Characterization of double-stranded-RNA-activated kinase that phosphorylates alpha subunit of eukaryotic initiation factor 2 (eIF-2 alpha) in reticulocyte lysates.

Authors:  D H Levin; R Petryshyn; I M London
Journal:  Proc Natl Acad Sci U S A       Date:  1980-02       Impact factor: 11.205

9.  Site-specific phosphorylation of the alpha subunit of eukaryotic initiation factor eIF-2 by the heme-regulated and double-stranded RNA-activated eIF-2 alpha kinases from rabbit reticulocyte lysates.

Authors:  V Ernst; D H Levin; A Leroux; I M London
Journal:  Proc Natl Acad Sci U S A       Date:  1980-03       Impact factor: 11.205

10.  Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites.

Authors:  Gabriela Arévalo-Pinzón; María González-González; Carlos Fernando Suárez; Hernando Curtidor; Javier Carabias-Sánchez; Antonio Muro; Joshua LaBaer; Manuel Alfonso Patarroyo; Manuel Fuentes
Journal:  Malar J       Date:  2018-07-17       Impact factor: 2.979
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.