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Literature DB >> 27181459

Site-directed spin labeling of proteins for distance measurements in vitro and in cells.

P Roser¹, M J Schmidt¹, M Drescher¹, D Summerer².

Abstract

Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy allows studying the structure, dynamics, and interactions of proteins via distance measurements in the nanometer range. We here give an overview of available spin labels, the strategies for their introduction into proteins, and the associated potentials for protein structural studies in vitro and in the context of living cells.

Mesh：

Substances：
Proteins
Spin Labels

Year: 2016 PMID： 27181459 DOI： 10.1039/c6ob00473c

Source DB: PubMed Journal: Org Biomol Chem ISSN： 1477-0520 Impact factor: 3.876

Keyword Cloud
Cited

24 in total

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4. Synthesis of structurally defined nitroxide spin-labeled glycolipids as useful probes for electron paramagnetic resonance (EPR) spectroscopy studies of cell surface glycans.

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Journal: Synthesis (Stuttg) Date: 2022-02-10 Impact factor: 2.969

Review 5. Paramagnetic Chemical Probes for Studying Biological Macromolecules.

Authors: Qing Miao; Christoph Nitsche; Henry Orton; Mark Overhand; Gottfried Otting; Marcellus Ubbink
Journal: Chem Rev Date: 2022-01-27 Impact factor: 72.087

6. Genetically Encoded Quinone Methides Enabling Rapid, Site-Specific, and Photocontrolled Protein Modification with Amine Reagents.

Authors: Jun Liu; Rujin Cheng; Ned Van Eps; Nanxi Wang; Takefumi Morizumi; Wei-Lin Ou; Paul C Klauser; Sharon Rozovsky; Oliver P Ernst; Lei Wang
Journal: J Am Chem Soc Date: 2020-09-25 Impact factor: 15.419

Site-directed spin labeling of proteins for distance measurements in vitro and in cells.

1. Reduction kinetics and electrochemistry of tetracarboxylate nitroxides.

Review 2. New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR.

3. Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy.

4. Synthesis of structurally defined nitroxide spin-labeled glycolipids as useful probes for electron paramagnetic resonance (EPR) spectroscopy studies of cell surface glycans.

Review 5. Paramagnetic Chemical Probes for Studying Biological Macromolecules.

6. Genetically Encoded Quinone Methides Enabling Rapid, Site-Specific, and Photocontrolled Protein Modification with Amine Reagents.

7. ESR Resolves the C Terminus Structure of the Ligand-free Human Glutathione S-Transferase A1-1.

8. Cleavage-Resistant Protein Labeling With Hydrophilic Trityl Enables Distance Measurements In-Cell.

9. Site-selective labeling and electron paramagnetic resonance studies of human cannabinoid receptor CB₂.

10. Synthesis and Electron Spin Relaxation of Tetracarboxylate Pyrroline Nitroxides.