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Literature DB >> 35171149

Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy.

Mohit Jaiswal¹, Trang T Tran¹, Jiatong Guo¹, Mingwei Zhou¹, Josefina Garcia Diaz¹, Gail E Fanucci¹, Zhongwu Guo¹.

Abstract

A novel method for spin labelling of sialoglycans on the cell surface is described. C9-Azido sialic acid was linked to glycans on live cells via CSTII-catalysed α2,3-sialylation utilizing azido-sialic acid nucleotide as a sialyl donor, which was followed by attachment of a spin label to the azide via click reaction. It enables the study of cell surface sialoglycans by EPR spectroscopy.

Entities: Chemical

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Year: 2022 PMID： 35171149 PMCID： PMC8885856 DOI： 10.1039/d1an02226a

Source DB: PubMed Journal: Analyst ISSN： 0003-2654 Impact factor: 4.616

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References

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Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy.

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