| Literature DB >> 27088312 |
Camilla Luni1,2, Stefano Giulitti1,2, Elena Serena1,2, Luca Ferrari1,2, Alessandro Zambon1,2, Onelia Gagliano1,2, Giovanni G Giobbe1,2, Federica Michielin1,2, Sebastian Knöbel3, Andreas Bosio3, Nicola Elvassore1,2.
Abstract
We report that the efficiency of reprogramming human somatic cells to induced pluripotent stem cells (hiPSCs) can be dramatically improved in a microfluidic environment. Microliter-volume confinement resulted in a 50-fold increase in efficiency over traditional reprogramming by delivery of synthetic mRNAs encoding transcription factors. In these small volumes, extracellular components of the TGF-β and other signaling pathways exhibited temporal regulation that appears critical to acquisition of pluripotency. The high quality and purity of the resulting hiPSCs (μ-hiPSCs) allowed direct differentiation into functional hepatocyte- and cardiomyocyte-like cells in the same platform without additional expansion.Entities:
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Year: 2016 PMID： 27088312 DOI： 10.1038/nmeth.3832
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547