Literature DB >> 27008876

One Small Step for the Gram Stain, One Giant Leap for Clinical Microbiology.

Richard B Thomson1.   

Abstract

The Gram stain is one of the most commonly performed tests in the clinical microbiology laboratory, yet it is poorly controlled and lacks standardization. It was once the best rapid test in microbiology, but it is no longer trusted by many clinicians. The publication by Samuel et al. (J. Clin. Microbiol. 54:1442-1447, 2016, http://dx.doi.org/10.1128/JCM.03066-15) is a start for those who want to evaluate and improve Gram stain performance. In an age of emerging rapid molecular results, is the Gram stain still relevant? How should clinical microbiologists respond to the call to reduce Gram stain error rates?
Copyright © 2016, American Society for Microbiology. All Rights Reserved.

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Year:  2016        PMID： 27008876      PMCID： PMC4879305          DOI： 10.1128/JCM.00303-16

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

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Authors:  S Nagendra; P Bourbeau; S Brecher; M Dunne; M LaRocco; G Doern
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2.  The Gram stain and the etiology of lobar pneumonia, an historical note.

Authors:  R AUSTRIAN
Journal:  Bacteriol Rev       Date:  1960-09

3.  Multicenter Assessment of Gram Stain Error Rates.

Authors:  Linoj P Samuel; Joan-Miquel Balada-Llasat; Amanda Harrington; Robert Cavagnolo
Journal:  J Clin Microbiol       Date:  2016-02-17       Impact factor: 5.948

4.  Errors in interpretation of Gram stains from positive blood cultures.

Authors:  Kenneth H Rand; Maria Tillan
Journal:  Am J Clin Pathol       Date:  2006-11       Impact factor: 2.493

5.  Concentrated gram stain smears prepared with a cytospin centrifuge.

Authors:  C J Shanholtzer; P J Schaper; L R Peterson
Journal:  J Clin Microbiol       Date:  1982-12       Impact factor: 5.948

6.  Diagnostic performance of a multiplex PCR assay for meningitis in an HIV-infected population in Uganda.

Authors:  Joshua Rhein; Nathan C Bahr; Andrew C Hemmert; Joann L Cloud; Satya Bellamkonda; Cody Oswald; Eric Lo; Henry Nabeta; Reuben Kiggundu; Andrew Akampurira; Abdu Musubire; Darlisha A Williams; David B Meya; David R Boulware
Journal:  Diagn Microbiol Infect Dis       Date:  2015-12-01       Impact factor: 2.803

7.  Evaluation of housestaff physicians' preparation and interpretation of sputum Gram stains for community-acquired pneumonia.

Authors:  M J Fine; J J Orloff; J D Rihs; R M Vickers; S Kominos; W N Kapoor; V C Arena; V L Yu
Journal:  J Gen Intern Med       Date:  1991 May-Jun       Impact factor: 5.128

8.  History of the specialty of infectious diseases in the United States.

Authors:  E H Kass
Journal:  Ann Intern Med       Date:  1987-05       Impact factor: 25.391

Review 9.  Pathogen identification of abscesses and cellulitis.

Authors:  H W Meislin
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10.  Diagnostic value of microscopic examination of Gram-stained sputum and sputum cultures in patients with bacteremic pneumococcal pneumonia.

Authors:  Daniel M Musher; Roberto Montoya; Anna Wanahita
Journal:  Clin Infect Dis       Date:  2004-07-01       Impact factor: 9.079
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  7 in total

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Journal:  J Clin Microbiol       Date:  2018-01-24       Impact factor: 5.948

2.  Waltzing around Sacred Cows on the Way to the Future.

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4.  Development of a standardized Gram stain procedure for bacteria and inflammatory cells using an automated staining instrument.

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Journal:  Microbiologyopen       Date:  2020-06-27       Impact factor: 3.139

5.  A Novel Improved Gram Staining Method Based on the Capillary Tube.

Authors:  Kong-Yang Wu; Tong-Xiang Yang
Journal:  Pol J Microbiol       Date:  2020-11-23

Review 6.  Application of Nanoparticle Technologies in the Combat against Anti-Microbial Resistance.

Authors:  Mayur Kumar; Anthony Curtis; Clare Hoskins
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Review 7.  Smartphone-based clinical diagnostics: towards democratization of evidence-based health care.

Authors:  I Hernández-Neuta; F Neumann; J Brightmeyer; T Ba Tis; N Madaboosi; Q Wei; A Ozcan; M Nilsson
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  7 in total

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