Ilwoo Park1, Cornelius von Morze1, Janine M Lupo1, Jan H Ardenkjaer-Larsen2,3, Achuta Kadambi4, Daniel B Vigneron1, Sarah J Nelson1,5. 1. Surbeck Laboratory of Advanced Imaging, Department of Radiology and Biomedical Imaging, University of California, San Francisco, California, USA. 2. GE Healthcare, Brøndby, Denmark. 3. Department of Electrical Engineering, Technical University of Denmark, Lyngby, Denmark. 4. Media Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. 5. Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California, USA.
Abstract
PURPOSE: Dissolution dynamic nuclear polarization (DNP) enables the acquisition of 13 C magnetic resonance data with a high sensitivity. Recently, metabolically inactive hyperpolarized 13 C-labeled compounds have shown to be potentially useful for perfusion imaging. The purpose of this study was to validate hyperpolarized perfusion imaging methods by comparing with conventional gadolinium (Gd)-based perfusion MRI techniques and pathology. METHODS: Dynamic 13 C data using metabolically inactive hyperpolarized bis-1,1-(hydroxymethyl)-[1-13 C]cyclopropane-d8 (HMCP) were obtained from an orthotopic human glioblastoma (GBM) model for the characterization of tumor perfusion and compared with standard Gd-based dynamic susceptibility contrast (DSC) MRI data and immunohistochemical analysis from resected brains. RESULTS: Distinct HMCP perfusion characteristics were observed within the GBM tumors compared with contralateral normal brain tissue. The perfusion parameters obtained from the hyperpolarized HMCP data in tumor were strongly correlated with normalized peak height measured from the DSC images. The results from immunohistochemical analysis supported these findings by showing a high level of vascular staining for tumor that exhibited high levels of hyperpolarized HMCP signal. CONCLUSION: The results from this study have demonstrated that hyperpolarized HMCP data can be used as an indicator of tumor perfusion in an orthotopic xenograft model for GBM. Magn Reson Med 77:841-847, 2017.
PURPOSE: Dissolution dynamic nuclear polarization (DNP) enables the acquisition of 13 C magnetic resonance data with a high sensitivity. Recently, metabolically inactive hyperpolarized 13 C-labeled compounds have shown to be potentially useful for perfusion imaging. The purpose of this study was to validate hyperpolarized perfusion imaging methods by comparing with conventional gadolinium (Gd)-based perfusion MRI techniques and pathology. METHODS: Dynamic 13 C data using metabolically inactive hyperpolarized bis-1,1-(hydroxymethyl)-[1-13 C]cyclopropane-d8 (HMCP) were obtained from an orthotopic humanglioblastoma (GBM) model for the characterization of tumor perfusion and compared with standard Gd-based dynamic susceptibility contrast (DSC) MRI data and immunohistochemical analysis from resected brains. RESULTS: Distinct HMCP perfusion characteristics were observed within the GBM tumors compared with contralateral normal brain tissue. The perfusion parameters obtained from the hyperpolarized HMCP data in tumor were strongly correlated with normalized peak height measured from the DSC images. The results from immunohistochemical analysis supported these findings by showing a high level of vascular staining for tumor that exhibited high levels of hyperpolarized HMCP signal. CONCLUSION: The results from this study have demonstrated that hyperpolarized HMCP data can be used as an indicator of tumor perfusion in an orthotopic xenograft model for GBM. Magn Reson Med 77:841-847, 2017.
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Authors: Ilwoo Park; Robert Bok; Tomoko Ozawa; Joanna J Phillips; C David James; Daniel B Vigneron; Sabrina M Ronen; Sarah J Nelson Journal: J Magn Reson Imaging Date: 2011-06 Impact factor: 4.813
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Authors: L S Hu; L C Baxter; D S Pinnaduwage; T L Paine; J P Karis; B G Feuerstein; K M Schmainda; A C Dueck; J Debbins; K A Smith; P Nakaji; J M Eschbacher; S W Coons; J E Heiserman Journal: AJNR Am J Neuroradiol Date: 2009-09-12 Impact factor: 3.825
Authors: Ilwoo Park; Simon Hu; Robert Bok; Tomoko Ozawa; Motokazu Ito; Joydeep Mukherjee; Joanna J Phillips; C David James; Russell O Pieper; Sabrina M Ronen; Daniel B Vigneron; Sarah J Nelson Journal: Magn Reson Med Date: 2012-07-31 Impact factor: 4.668