Literature DB >> 26835505

Quantitative assessments of glycolysis from single cells.

Young Shik Shin1, Jungwoo Kim2, Dazy Johnson3, Alex A Dooraghi4, Wilson X Mai3, Lisa Ta3, Arion F Chatziioannou4, Michael E Phelps5, David A Nathanson3, James R Heath1.   

Abstract

The most common positron emission tomography (PET) radio-labeled probe for molecular diagnostics in patient care and research is the glucose analog, 2-deoxy-2-[F-18]fluoro-D-glucose (18F-FDG). We report on an integrated microfluidics-chip/beta particle imaging system for in vitro18F-FDG radioassays of glycolysis with single cell resolution. We investigated the kinetic responses of single glioblastoma cancer cells to targeted inhibitors of receptor tyrosine kinase signaling. Further, we find a weak positive correlation between cell size and rate of glycolysis.

Entities:  

Year:  2015        PMID： 26835505      PMCID： PMC4728151          DOI： 10.1142/S2339547815200058

Source DB:  PubMed          Journal:  Technology (Singap World Sci)


  16 in total

1.  Microfluidic device for single-cell analysis.

Authors:  Aaron R Wheeler; William R Throndset; Rebecca J Whelan; Andrew M Leach; Richard N Zare; Yish Hann Liao; Kevin Farrell; Ian D Manger; Antoine Daridon
Journal:  Anal Chem       Date:  2003-07-15       Impact factor: 6.986

2.  Production of radioactive quality assurance phantoms using a standard inkjet printer.

Authors:  J A van Staden; H du Raan; M G Lötter; A van Aswegen; C P Herbst
Journal:  Phys Med Biol       Date:  2007-07-03       Impact factor: 3.609

Review 3.  Single-cell metabolomics: analytical and biological perspectives.

Authors:  R Zenobi
Journal:  Science       Date:  2013-12-06       Impact factor: 47.728

4.  Betabox: a beta particle imaging system based on a position sensitive avalanche photodiode.

Authors:  A A Dooraghi; N T Vu; R W Silverman; R Farrell; K S Shah; J Wang; J R Heath; A F Chatziioannou
Journal:  Phys Med Biol       Date:  2013-05-08       Impact factor: 3.609

5.  Chemical methods for the simultaneous quantitation of metabolites and proteins from single cells.

Authors:  Min Xue; Wei Wei; Yapeng Su; Jungwoo Kim; Young Shik Shin; Wilson X Mai; David A Nathanson; James R Heath
Journal:  J Am Chem Soc       Date:  2015-03-19       Impact factor: 15.419

6.  Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane).

Authors:  D C Duffy; J C McDonald; O J Schueller; G M Whitesides
Journal:  Anal Chem       Date:  1998-12-01       Impact factor: 6.986

7.  Single-cell RNA-seq highlights intratumoral heterogeneity in primary glioblastoma.

Authors:  Anoop P Patel; Itay Tirosh; John J Trombetta; Alex K Shalek; Shawn M Gillespie; Hiroaki Wakimoto; Daniel P Cahill; Brian V Nahed; William T Curry; Robert L Martuza; David N Louis; Orit Rozenblatt-Rosen; Mario L Suvà; Aviv Regev; Bradley E Bernstein
Journal:  Science       Date:  2014-06-12       Impact factor: 47.728

Review 8.  Why do cancers have high aerobic glycolysis?

Authors:  Robert A Gatenby; Robert J Gillies
Journal:  Nat Rev Cancer       Date:  2004-11       Impact factor: 60.716

9.  Dissecting genomic diversity, one cell at a time.

Authors:  Paul C Blainey; Stephen R Quake
Journal:  Nat Methods       Date:  2014-01       Impact factor: 28.547

10.  Fast metabolic response to drug intervention through analysis on a miniaturized, highly integrated molecular imaging system.

Authors:  Jun Wang; Kiwook Hwang; Daniel Braas; Alex Dooraghi; David Nathanson; Dean O Campbell; Yuchao Gu; Troy Sandberg; Paul Mischel; Caius Radu; Arion F Chatziioannou; Michael E Phelps; Heather Christofk; James R Heath
Journal:  J Nucl Med       Date:  2013-08-26       Impact factor: 10.057
View more
  1 in total

Review 1.  Recent Progress toward Microfluidic Quality Control Testing of Radiopharmaceuticals.

Authors:  Noel S Ha; Saman Sadeghi; R Michael van Dam
Journal:  Micromachines (Basel)       Date:  2017-11-21       Impact factor: 2.891
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.