Literature DB >> 26806645

Backbone assignment for minimal protein amounts of low structural homogeneity in the absence of deuteration.

ShengQi Xiang1, Jacek Biernat2, Eckhard Mandelkow2, Stefan Becker1, Rasmus Linser1.   

Abstract

NMR characterization of many proteins is limited by low expression, hurdles for deuteration, and poor sample homogeneity. We introduce a set of high-dimensionality proton-detected experiments developed for unambiguous resonance assignments of such proteins, which we could successfully apply to a 1 mg amount of non-deuterated Tau paired helical filaments.

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Year:  2016        PMID： 26806645     DOI： 10.1039/c5cc09160h

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  11 in total

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Authors:  Patrick C A van der Wel
Journal:  Solid State Nucl Magn Reson       Date:  2017-10-04       Impact factor: 2.293

2.  High-Sensitivity Detection of Nanometer 1H-19F Distances for Protein Structure Determination by 1H-Detected Fast MAS NMR.

Authors:  Alexander A Shcherbakov; Venkata Shiva Mandala; Mei Hong
Journal:  J Phys Chem B       Date:  2019-05-08       Impact factor: 2.991

3.  Detection of side-chain proton resonances of fully protonated biosolids in nano-litre volumes by magic angle spinning solid-state NMR.

Authors:  James Tolchard; Manoj Kumar Pandey; Mélanie Berbon; Abdelmajid Noubhani; Sven J Saupe; Yusuke Nishiyama; Birgit Habenstein; Antoine Loquet
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4.  Two decades of progress in structural and dynamic studies of amyloids by solid-state NMR.

Authors:  Christopher P Jaroniec
Journal:  J Magn Reson       Date:  2019-07-09       Impact factor: 2.229

Review 5.  1H-Detected Biomolecular NMR under Fast Magic-Angle Spinning.

Authors:  Tanguy Le Marchand; Tobias Schubeis; Marta Bonaccorsi; Piotr Paluch; Daniela Lalli; Andrew J Pell; Loren B Andreas; Kristaps Jaudzems; Jan Stanek; Guido Pintacuda
Journal:  Chem Rev       Date:  2022-05-10       Impact factor: 72.087

6.  Gd3+-chelated lipid accelerates solid-state NMR spectroscopy of seven-transmembrane proteins.

Authors:  Chang Liu; Jing Liu; Xiaojun Xu; ShengQi Xiang; Shenlin Wang
Journal:  J Biomol NMR       Date:  2017-05-30       Impact factor: 2.835

7.  Direct amide 15N to 13C transfers for solid-state assignment experiments in deuterated proteins.

Authors:  Alons Lends; Francesco Ravotti; Giorgia Zandomeneghi; Anja Böckmann; Matthias Ernst; Beat H Meier
Journal:  J Biomol NMR       Date:  2018-09-11       Impact factor: 2.835

8.  Structure of fully protonated proteins by proton-detected magic-angle spinning NMR.

Authors:  Loren B Andreas; Kristaps Jaudzems; Jan Stanek; Daniela Lalli; Andrea Bertarello; Tanguy Le Marchand; Diane Cala-De Paepe; Svetlana Kotelovica; Inara Akopjana; Benno Knott; Sebastian Wegner; Frank Engelke; Anne Lesage; Lyndon Emsley; Kaspars Tars; Torsten Herrmann; Guido Pintacuda
Journal:  Proc Natl Acad Sci U S A       Date:  2016-08-03       Impact factor: 11.205

9.  Proton-Detected Solid-State NMR of the Cell-Free Synthesized α-Helical Transmembrane Protein NS4B from Hepatitis C Virus.

Authors:  Vlastimil Jirasko; Nils-Alexander Lakomek; Susanne Penzel; Marie-Laure Fogeron; Ralf Bartenschlager; Beat H Meier; Anja Böckmann
Journal:  Chembiochem       Date:  2020-02-20       Impact factor: 3.164

10.  1 H-Detected Solid-State NMR Studies of Water-Inaccessible Proteins In Vitro and In Situ.

Authors:  João Medeiros-Silva; Deni Mance; Mark Daniëls; Shehrazade Jekhmane; Klaartje Houben; Marc Baldus; Markus Weingarth
Journal:  Angew Chem Int Ed Engl       Date:  2016-09-27       Impact factor: 15.336
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