Literature DB >> 26739156

Performance and Verification of a Real-Time PCR Assay Targeting the gyrA Gene for Prediction of Ciprofloxacin Resistance in Neisseria gonorrhoeae.

P Hemarajata1, S Yang1, O O Soge2, R M Humphries3, J D Klausner4.   

Abstract

In the United States, 19.2% of Neisseria gonorrhoeae isolates are resistant to ciprofloxacin. We evaluated a real-time PCR assay to predict ciprofloxacin susceptibility using residual DNA from the Roche Cobas 4800 CT/NG assay. The results of the assay were 100% concordant with agar dilution susceptibility test results for 100 clinical isolates. Among 76 clinical urine and swab specimens positive for N. gonorrhoeae by the Cobas assay, 71% could be genotyped. The test took 1.5 h to perform, allowing the physician to receive results in time to make informed clinical decisions.
Copyright © 2016, American Society for Microbiology. All Rights Reserved.

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Year:  2016        PMID： 26739156      PMCID： PMC4767994          DOI： 10.1128/JCM.03032-15

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

1.  Molecular analysis of antimicrobial resistance mechanisms in Neisseria gonorrhoeae isolates from Ontario, Canada.

Authors:  Vanessa G Allen; David J Farrell; Anuradha Rebbapragada; Jingyuan Tan; Nathalie Tijet; Stephen J Perusini; Lynn Towns; Stephen Lo; Donald E Low; Roberto G Melano
Journal:  Antimicrob Agents Chemother       Date:  2010-11-22       Impact factor: 5.191

2.  Update to CDC's Sexually transmitted diseases treatment guidelines, 2010: oral cephalosporins no longer a recommended treatment for gonococcal infections.

Authors: 
Journal:  MMWR Morb Mortal Wkly Rep       Date:  2012-08-10       Impact factor: 17.586

3.  Cephalosporin resistance in Neisseria gonorrhoeae infections.

Authors:  Jeffrey D Klausner; Peter Kerndt
Journal:  JAMA       Date:  2013-05-15       Impact factor: 56.272

Review 4.  The ticking time bomb: escalating antibiotic resistance in Neisseria gonorrhoeae is a public health disaster in waiting.

Authors:  David M Whiley; Namraj Goire; Monica M Lahra; Basil Donovan; Athena E Limnios; Michael D Nissen; Theo P Sloots
Journal:  J Antimicrob Chemother       Date:  2012-05-17       Impact factor: 5.790

5.  Determination of Neisseria gonorrhoeae susceptibility to ciprofloxacin in clinical specimens from men using a real-time PCR assay.

Authors:  M P Magooa; E E Müller; L Gumede; D A Lewis
Journal:  Int J Antimicrob Agents       Date:  2013-04-24       Impact factor: 5.283

6.  Update to CDC's sexually transmitted diseases treatment guidelines, 2006: fluoroquinolones no longer recommended for treatment of gonococcal infections.

Authors: 
Journal:  MMWR Morb Mortal Wkly Rep       Date:  2007-04-13       Impact factor: 17.586

7.  Real-time PCR assay for detection of quinolone-resistant Neisseria gonorrhoeae in urine samples.

Authors:  Mark J Siedner; Mark Pandori; Lina Castro; Pennan Barry; William L H Whittington; Sally Liska; Jeffrey D Klausner
Journal:  J Clin Microbiol       Date:  2007-01-31       Impact factor: 5.948

8.  Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil.

Authors:  Aline A Uehara; Efigênia L T Amorin; Maria de Fátima Ferreira; Claudia F Andrade; Maysa B M Clementino; Ivano de Filippis; Felipe P G Neves; Tatiana de C A Pinto; Lúcia M Teixeira; Marcia Giambiagi-Demarval; Sérgio E L Fracalanzza
Journal:  J Clin Microbiol       Date:  2011-10-05       Impact factor: 5.948

9.  Protocol for the molecular detection of antibiotic resistance mechanisms in Neisseria gonorrhoeae.

Authors:  Namraj Goire; Theo P Sloots; Michael D Nissen; David M Whiley
Journal:  Methods Mol Biol       Date:  2012

Review 10.  Chlamydia and gonorrhea screening in United States emergency departments.

Authors:  Wiley D Jenkins; Whitney Zahnd; Regina Kovach; Patricia Kissinger
Journal:  J Emerg Med       Date:  2012-10-26       Impact factor: 1.484
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  22 in total

1.  The Frequency of Discordant Gyrase A Genotypes Among Cases of Multiple Neisseria gonorrhoeae Infections at Different Anatomic Sites.

Authors:  Lao-Tzu Allan-Blitz; Olivia L Ellis; Robert Bolan; Peera Hemarajata; Romney M Humphries; Akbar Shahkolahi; Mark McGrath; Jeffrey D Klausner
Journal:  Sex Transm Dis       Date:  2019-01       Impact factor: 2.830

2.  Codon 91 Gyrase A Testing Is Necessary and Sufficient to Predict Ciprofloxacin Susceptibility in Neisseria gonorrhoeae.

Authors:  Lao-Tzu Allan-Blitz; Jeffrey D Klausner
Journal:  J Infect Dis       Date:  2017-02-01       Impact factor: 5.226

3.  Real-Time PCR Targeting the penA Mosaic XXXIV Type for Prediction of Extended-Spectrum-Cephalosporin Susceptibility in Clinical Neisseria gonorrhoeae Isolates.

Authors:  L K Wong; P Hemarajata; O O Soge; R M Humphries; J D Klausner
Journal:  Antimicrob Agents Chemother       Date:  2017-10-24       Impact factor: 5.191

4.  Molecular Characterization of Markers Associated With Antimicrobial Resistance in Neisseria gonorrhoeae Identified From Residual Clinical Samples.

Authors:  Johan H Melendez; Justin Hardick; Mathilda Barnes; Perry Barnes; Christopher D Geddes; Charlotte A Gaydos
Journal:  Sex Transm Dis       Date:  2018-05       Impact factor: 2.830

5.  Ciprofloxacin May be Efficacious in Treating Wild-Type Gyrase A Genotype Neisseria gonorrhoeae Infections.

Authors:  Lao-Tzu Allan-Blitz; Peera Hemarajata; Romney M Humphries; Mabel Kimble; Sam Elias; Jeffrey D Klausner
Journal:  Sex Transm Dis       Date:  2018-04       Impact factor: 2.830

6.  A Cost Analysis of Gyrase A Testing and Targeted Ciprofloxacin Therapy Versus Recommended 2-Drug Therapy for Neisseria gonorrhoeae Infection.

Authors:  Lao-Tzu Allan-Blitz; Peera Hemarajata; Romney M Humphries; Adriane Wynn; Eddy R Segura; Jeffrey D Klausner
Journal:  Sex Transm Dis       Date:  2018-02       Impact factor: 2.830

7.  Implementation of a Rapid Genotypic Assay to Promote Targeted Ciprofloxacin Therapy of Neisseria gonorrhoeae in a Large Health System.

Authors:  Lao-Tzu Allan-Blitz; Romney M Humphries; Peera Hemarajata; Ashima Bhatti; Mark W Pandori; Mark J Siedner; Jeffrey D Klausner
Journal:  Clin Infect Dis       Date:  2017-05-01       Impact factor: 9.079

8.  A multisite implementation of a real-time polymerase chain reaction assay to predict ciprofloxacin susceptibility in Neisseria gonorrhoeae.

Authors:  Olivia Ellis; Peera Hemarajata; Akbar Shahkolahi; Godfred Masinde; Kerry Buchs; Romney M Humphries; Jeffrey D Klausner
Journal:  Diagn Microbiol Infect Dis       Date:  2019-01-14       Impact factor: 2.803

9.  Technological Solutions to Address Drug-Resistant Neisseria gonorrhoeae.

Authors:  Claire C Bristow; Huan Dong; Jeffrey D Klausner
Journal:  Emerg Infect Dis       Date:  2016-05       Impact factor: 6.883

10.  Genomic Epidemiology of Gonococcal Resistance to Extended-Spectrum Cephalosporins, Macrolides, and Fluoroquinolones in the United States, 2000-2013.

Authors:  Yonatan H Grad; Simon R Harris; Robert D Kirkcaldy; Anna G Green; Debora S Marks; Stephen D Bentley; David Trees; Marc Lipsitch
Journal:  J Infect Dis       Date:  2016-09-16       Impact factor: 5.226
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