Literature DB >> 28848021

Real-Time PCR Targeting the penA Mosaic XXXIV Type for Prediction of Extended-Spectrum-Cephalosporin Susceptibility in Clinical Neisseria gonorrhoeae Isolates.

L K Wong1, P Hemarajata2, O O Soge3, R M Humphries4, J D Klausner5.   

Abstract

Neisseria gonorrhoeae isolates with decreased susceptibility to extended-spectrum cephalosporins (ESCs) are increasing. We developed an assay to predict N. gonorrhoeae susceptibility to ESCs by targeting penA mosaic XXXIV, an allele prevalent among U.S. isolates with elevated ESC MICs. The assay was 97% sensitive and 100% specific for predicting at least one ESC MIC above the CDC alert value among clinical isolates, and it could be multiplexed with a previously validated gyrA PCR to predict ciprofloxacin susceptibility.
Copyright © 2017 American Society for Microbiology.

Entities:  

Keywords:  Neisseria gonorrhoeae; antibiotic resistance; antimicrobial resistance; ceftriaxone; cephalosporin; extended-spectrum cephalosporins; gonorrhea; molecular methods

Mesh：

Substances：

Year:  2017        PMID： 28848021      PMCID： PMC5655115          DOI： 10.1128/AAC.01339-17

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  30 in total

Review 1.  Emergence of multidrug-resistant, extensively drug-resistant and untreatable gonorrhea.

Authors:  Magnus Unemo; Robert A Nicholas
Journal:  Future Microbiol       Date:  2012-12       Impact factor: 3.165

2.  High-level cefixime- and ceftriaxone-resistant Neisseria gonorrhoeae in France: novel penA mosaic allele in a successful international clone causes treatment failure.

Authors:  Magnus Unemo; Daniel Golparian; Robert Nicholas; Makoto Ohnishi; Anne Gallay; Patrice Sednaoui
Journal:  Antimicrob Agents Chemother       Date:  2011-12-12       Impact factor: 5.191

3.  Novel penA mutations identified in Neisseria gonorrhoeae with decreased susceptibility to ceftriaxone isolated between 2000 and 2014 in Japan.

Authors:  Kensaku Seike; Mitsuru Yasuda; Kyoko Hatazaki; Kosuke Mizutani; Kazuya Yuhara; Yasuhisa Ito; Yoshinori Fujimoto; Shin Ito; Tomohiro Tsuchiya; Shigeaki Yokoi; Masahiro Nakano; Takashi Deguchi
Journal:  J Antimicrob Chemother       Date:  2016-05-13       Impact factor: 5.790

4.  Antimicrobial Susceptibility of Neisseria gonorrhoeae in Japan from 2000 to 2015.

Authors:  Mitsuru Yasuda; Kyoko Hatazaki; Shin Ito; Masataka Kitanohara; Mutsumasa Yoh; Munekado Kojima; Harunori Narita; Akira Kido; Kazutoyo Miyata; Takashi Deguch
Journal:  Sex Transm Dis       Date:  2017-03       Impact factor: 2.830

Review 5.  Molecular tests for the detection of antimicrobial resistant Neisseria gonorrhoeae: when, where, and how to use?

Authors:  Nicola Low; Magnus Unemo
Journal:  Curr Opin Infect Dis       Date:  2016-02       Impact factor: 4.915

Review 6.  The use of cephalosporins for gonorrhea: the impending problem of resistance.

Authors:  Pennan M Barry; Jeffrey D Klausner
Journal:  Expert Opin Pharmacother       Date:  2009-03       Impact factor: 3.889

7.  Performance and Verification of a Real-Time PCR Assay Targeting the gyrA Gene for Prediction of Ciprofloxacin Resistance in Neisseria gonorrhoeae.

Authors:  P Hemarajata; S Yang; O O Soge; R M Humphries; J D Klausner
Journal:  J Clin Microbiol       Date:  2016-01-06       Impact factor: 5.948

8.  Improvements to PATRIC, the all-bacterial Bioinformatics Database and Analysis Resource Center.

Authors:  Alice R Wattam; James J Davis; Rida Assaf; Sébastien Boisvert; Thomas Brettin; Christopher Bun; Neal Conrad; Emily M Dietrich; Terry Disz; Joseph L Gabbard; Svetlana Gerdes; Christopher S Henry; Ronald W Kenyon; Dustin Machi; Chunhong Mao; Eric K Nordberg; Gary J Olsen; Daniel E Murphy-Olson; Robert Olson; Ross Overbeek; Bruce Parrello; Gordon D Pusch; Maulik Shukla; Veronika Vonstein; Andrew Warren; Fangfang Xia; Hyunseung Yoo; Rick L Stevens
Journal:  Nucleic Acids Res       Date:  2016-11-29       Impact factor: 16.971

9.  PATRIC, the bacterial bioinformatics database and analysis resource.

Authors:  Alice R Wattam; David Abraham; Oral Dalay; Terry L Disz; Timothy Driscoll; Joseph L Gabbard; Joseph J Gillespie; Roger Gough; Deborah Hix; Ronald Kenyon; Dustin Machi; Chunhong Mao; Eric K Nordberg; Robert Olson; Ross Overbeek; Gordon D Pusch; Maulik Shukla; Julie Schulman; Rick L Stevens; Daniel E Sullivan; Veronika Vonstein; Andrew Warren; Rebecca Will; Meredith J C Wilson; Hyun Seung Yoo; Chengdong Zhang; Yan Zhang; Bruno W Sobral
Journal:  Nucleic Acids Res       Date:  2013-11-12       Impact factor: 16.971

10.  Neisseria gonorrhoeae and extended-spectrum cephalosporins in California: surveillance and molecular detection of mosaic penA.

Authors:  Severin Gose; Duylinh Nguyen; Daniella Lowenberg; Michael Samuel; Heidi Bauer; Mark Pandori
Journal:  BMC Infect Dis       Date:  2013-12-04       Impact factor: 3.090
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  7 in total

1.  Six penA Codons Accurately and Reliably Predict Cefixime-Decreased Susceptibility in Neisseria gonorrhoeae.

Authors:  Xiaomeng Deng; Jeffrey D Klausner
Journal:  J Infect Dis       Date:  2020-02-18       Impact factor: 5.226

2.  A multisite implementation of a real-time polymerase chain reaction assay to predict ciprofloxacin susceptibility in Neisseria gonorrhoeae.

Authors:  Olivia Ellis; Peera Hemarajata; Akbar Shahkolahi; Godfred Masinde; Kerry Buchs; Romney M Humphries; Jeffrey D Klausner
Journal:  Diagn Microbiol Infect Dis       Date:  2019-01-14       Impact factor: 2.803

Review 3.  Bridging the gap between development of point-of-care nucleic acid testing and patient care for sexually transmitted infections.

Authors:  Kuangwen Hsieh; Johan H Melendez; Charlotte A Gaydos; Tza-Huei Wang
Journal:  Lab Chip       Date:  2022-02-01       Impact factor: 7.517

4.  Using the genetic characteristics of Neisseria gonorrhoeae strains with decreased susceptibility to cefixime to develop a molecular assay to predict cefixime susceptibility.

Authors:  Xiaomeng Deng; Lao-Tzu Allan-Blitz; Jeffrey D Klausner
Journal:  Sex Health       Date:  2019-09       Impact factor: 2.706

5.  Genotypic and Phenotypic Characterization of Antimicrobial Resistance in Neisseria gonorrhoeae: a Cross-Sectional Study of Isolates Recovered from Routine Urine Cultures in a High-Incidence Setting.

Authors:  Adam L Bailey; Robert F Potter; Meghan A Wallace; Caitlin Johnson; Gautam Dantas; C A Burnham
Journal:  mSphere       Date:  2019-07-24       Impact factor: 4.389

6.  Why Can't We Just Use PCR? The Role of Genotypic versus Phenotypic Testing for Antimicrobial Resistance Testing.

Authors:  Jennifer Dien Bard; Francesca Lee
Journal:  Clin Microbiol Newsl       Date:  2018-05-22

7.  Surfactant-enhanced DNA accessibility to nuclease accelerates phenotypic β-lactam antibiotic susceptibility testing of Neisseria gonorrhoeae.

Authors:  Emily S Savela; Nathan G Schoepp; Matthew M Cooper; Justin C Rolando; Jeffrey D Klausner; Olusegun O Soge; Rustem F Ismagilov
Journal:  PLoS Biol       Date:  2020-03-19       Impact factor: 8.029
  7 in total

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