Literature DB >> 26705325

Principles of cryo-EM single-particle image processing.

Fred J Sigworth1.   

Abstract

Single-particle reconstruction is the process by which 3D density maps are obtained from a set of low-dose cryo-EM images of individual macromolecules. This review considers the fundamental principles of this process and the steps in the overall workflow for single-particle image processing. Also considered are the limits that image signal-to-noise ratio places on resolution and the distinguishing of heterogeneous particle populations.
© The Author 2015. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Keywords:  3D reconstruction; SNR; noise

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Year:  2015        PMID: 26705325      PMCID: PMC4749045          DOI: 10.1093/jmicro/dfv370

Source DB:  PubMed          Journal:  Microscopy (Oxf)        ISSN: 2050-5698            Impact factor:   1.571


  39 in total

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