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Literature DB >> 26684483

Light-Activated Reversible Imine Isomerization: Towards a Photochromic Protein Switch.

Tetyana Berbasova¹, Elizabeth M Santos¹, Meisam Nosrati¹, Chrysoula Vasileiou¹, James H Geiger¹, Babak Borhan¹.

Abstract

Mutants of cellular retinoic acid-binding protein II (CRABPII), engineered to bind all-trans-retinal as an iminium species, demonstrate photochromism upon irradiation with light at different wavelengths. UV light irradiation populates the cis-imine geometry, which has a high pKa , leading to protonation of the imine and subsequent "turn-on" of color. Yellow light irradiation yields the trans-imine isomer, which has a depressed pKa , leading to loss of color because the imine is not protonated. The protein-bound retinylidene chromophore undergoes photoinduced reversible interconversion between the colored and uncolored species, with excellent fatigue resistance.

Entities: CellLine Chemical Disease Gene Mutation Species

Keywords: imines; isomerization; photochemistry; protein engineering; retinal

Mesh：

Substances：

Year: 2016 PMID： 26684483 PMCID： PMC4835339 DOI： 10.1002/cbic.201500613

Source DB: PubMed Journal: Chembiochem ISSN： 1439-4227 Impact factor: 3.164

54 in total

1. Bacteriorhodopsin as a Photochromic Retinal Protein for Optical Memories.

Authors: Norbert Hampp
Journal: Chem Rev Date: 2000-05-10 Impact factor: 60.622

2. Bacteriorhodopsin: a light-driven proton pump in Halobacterium Halobium.

Authors: R H Lozier; R A Bogomolni; W Stoeckenius
Journal: Biophys J Date: 1975-09 Impact factor: 4.033

Review 3. Chemical dynamics in proteins: the photoisomerization of retinal in bacteriorhodopsin.

Authors: F Gai; K C Hasson; J C McDonald; P A Anfinrud
Journal: Science Date: 1998-03-20 Impact factor: 47.728

Review 4. Bacteriorhodopsin and the purple membrane of halobacteria.

Authors: W Stoeckenius; R H Lozier; R A Bogomolni
Journal: Biochim Biophys Acta Date: 1979-03-14

Review 5. Bacteriorhodopsin: a high-resolution structural view of vectorial proton transport.

Authors: Richard Neutze; Eva Pebay-Peyroula; Karl Edman; Antoine Royant; Javier Navarro; Ehud M Landau
Journal: Biochim Biophys Acta Date: 2002-10-11

6. Estimated acid dissociation constants of the Schiff base, Asp-85, and Arg-82 during the bacteriorhodopsin photocycle.

Authors: L S Brown; L Bonet; R Needleman; J K Lanyi
Journal: Biophys J Date: 1993-07 Impact factor: 4.033

7. Elucidating the exact role of engineered CRABPII residues for the formation of a retinal protonated Schiff base.

Authors: Chrysoula Vasileiou; Wenjing Wang; Xiaofei Jia; Kin Sing Stephen Lee; Camille T Watson; James H Geiger; Babak Borhan
Journal: Proteins Date: 2009-12

8. Dissection of the critical binding determinants of cellular retinoic acid binding protein II by mutagenesis and fluorescence binding assay.

Authors: Chrysoula Vasileiou; Kin Sing Stephen Lee; Rachael M Crist; Soheila Vaezeslami; Sarah M Goins; James H Geiger; Babak Borhan
Journal: Proteins Date: 2009-08-01

Review 9. Comparative analysis of GPCR crystal structures.

Authors: David T Lodowski; Thomas E Angel; Krzysztof Palczewski
Journal: Photochem Photobiol Date: 2009-01-19 Impact factor: 3.421

10. Toward an understanding of the retinal chromophore in rhodopsin mimics.

Authors: Mark M Huntress; Samer Gozem; Konstantin R Malley; Askat E Jailaubekov; Chrysoula Vasileiou; Mikas Vengris; James H Geiger; Babak Borhan; Igor Schapiro; Delmar S Larsen; Massimo Olivucci
Journal: J Phys Chem B Date: 2013-08-26 Impact factor: 2.991

3 in total