Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A Near-Infrared Photoswitchable Protein-Fluorophore Tag for No-Wash Live Cell Imaging.

Literature DB >> 30311335

A Near-Infrared Photoswitchable Protein-Fluorophore Tag for No-Wash Live Cell Imaging.

Wei Sheng¹, Setare Tahmasebi Nick¹, Elizabeth M Santos², Xinliang Ding¹, Jun Zhang¹, Chrysoula Vasileiou¹, James H Geiger¹, Babak Borhan¹.

Abstract

FR-1V, a fluorene-based aldehydic chromophore, binds its target protein as an imine to yield a highly bathochromic pigment, CF-2, a prototypic protein-dye tagging system whose NIR emission can be spatiotemporally switched ON by rapid UV-light activation. This is achieved through photoisomerization of the imine and its subsequent protonation. We demonstrate a no-wash protocol for live cell imaging of subcellular compartments in a variety of mammalian cell lines with minimal fluorescence background.

Entities: CellLine Chemical Disease Gene Species

Keywords: NIR fluorescence; confocal imaging; fluorene; photoswitch; red-shifted fluorophores

Mesh：

Substances：
Fluorescent Dyes
Proteins

Year: 2018 PMID： 30311335 PMCID： PMC7796701 DOI： 10.1002/anie.201810065

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

27 in total

1. A clearer vision for in vivo imaging.

Authors: R Weissleder
Journal: Nat Biotechnol Date: 2001-04 Impact factor: 54.908

2. A fluorophore ligase for site-specific protein labeling inside living cells.

Authors: Chayasith Uttamapinant; Katharine A White; Hemanta Baruah; Samuel Thompson; Marta Fernández-Suárez; Sujiet Puthenveetil; Alice Y Ting
Journal: Proc Natl Acad Sci U S A Date: 2010-06-07 Impact factor: 11.205

3. Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo.

Authors: Marie-Aude Plamont; Emmanuelle Billon-Denis; Sylvie Maurin; Carole Gauron; Frederico M Pimenta; Christian G Specht; Jian Shi; Jérôme Quérard; Buyan Pan; Julien Rossignol; Karine Moncoq; Nelly Morellet; Michel Volovitch; Ewen Lescop; Yong Chen; Antoine Triller; Sophie Vriz; Thomas Le Saux; Ludovic Jullien; Arnaud Gautier
Journal: Proc Natl Acad Sci U S A Date: 2015-12-28 Impact factor: 11.205

Review 4. Small-molecule-based protein-labeling technology in live cell studies: probe-design concepts and applications.

Authors: Shin Mizukami; Yuichiro Hori; Kazuya Kikuchi
Journal: Acc Chem Res Date: 2013-08-08 Impact factor: 22.384

5. Near-infrared fluorescent proteins.

Authors: Dmitry Shcherbo; Irina I Shemiakina; Anastasiya V Ryabova; Kathryn E Luker; Bradley T Schmidt; Ekaterina A Souslova; Tatiana V Gorodnicheva; Lydia Strukova; Konstantin M Shidlovskiy; Olga V Britanova; Andrey G Zaraisky; Konstantin A Lukyanov; Victor B Loschenov; Gary D Luker; Dmitriy M Chudakov
Journal: Nat Methods Date: 2010-09-05 Impact factor: 28.547

6. Biomedical applications of tetrazine cycloadditions.

Authors: Neal K Devaraj; Ralph Weissleder
Journal: Acc Chem Res Date: 2011-05-31 Impact factor: 22.384

7. Bistetrazine-Cyanines as Double-Clicking Fluorogenic Two-Point Binder or Crosslinker Probes.

Authors: Attila Kormos; Christine Koehler; Eszter A Fodor; Zsófia R Rutkai; Madison E Martin; Gábor Mező; Edward A Lemke; Péter Kele
Journal: Chemistry Date: 2018-05-29 Impact factor: 5.236

8. Fluorescent labeling of tetracysteine-tagged proteins in intact cells.

Authors: Carsten Hoffmann; Guido Gaietta; Alexander Zürn; Stephen R Adams; Sonia Terrillon; Mark H Ellisman; Roger Y Tsien; Martin J Lohse
Journal: Nat Protoc Date: 2010-09-23 Impact factor: 13.491

9. Tuning the electronic absorption of protein-embedded all-trans-retinal.

Authors: Wenjing Wang; Zahra Nossoni; Tetyana Berbasova; Camille T Watson; Ipek Yapici; Kin Sing Stephen Lee; Chrysoula Vasileiou; James H Geiger; Babak Borhan
Journal: Science Date: 2012-12-07 Impact factor: 47.728

10. Segregation of molecules at cell division reveals native protein localization.

Authors: Dirk Landgraf; Burak Okumus; Peter Chien; Tania A Baker; Johan Paulsson
Journal: Nat Methods Date: 2012-04-08 Impact factor: 28.547

8 in total

1. Engineering of a Red Fluorogenic Protein/Merocyanine Complex for Live-Cell Imaging.

Authors: Elizabeth M Santos; Tetyana Berbasova; Wenjing Wang; Rahele Esmatpour Salmani; Wei Sheng; Chrysoula Vasileiou; James H Geiger; Babak Borhan
Journal: Chembiochem Date: 2019-11-13 Impact factor: 3.164

2. Human Cellular Retinol Binding Protein II Forms a Domain-Swapped Trimer Representing a Novel Fold and a New Template for Protein Engineering.

Authors: Alireza Ghanbarpour; Elizabeth M Santos; Cody Pinger; Zahra Assar; Seyedmehdi Hossaini Nasr; Chrysoula Vasileiou; Dana Spence; Babak Borhan; James H Geiger
Journal: Chembiochem Date: 2020-08-14 Impact factor: 3.164

8. Intra Q-body: an antibody-based fluorogenic probe for intracellular proteins that allows live cell imaging and sorting.

Authors: Yancen Dai; Yuko Sato; Bo Zhu; Tetsuya Kitaguchi; Hiroshi Kimura; Farid J Ghadessy; Hiroshi Ueda
Journal: Chem Sci Date: 2022-08-01 Impact factor: 9.969