Literature DB >> 26524241

Thermal proteome profiling monitors ligand interactions with cellular membrane proteins.

Friedrich B M Reinhard1, Dirk Eberhard1, Thilo Werner1, Holger Franken1, Dorothee Childs1,2, Carola Doce1, Maria Fälth Savitski1, Wolfgang Huber2, Marcus Bantscheff1, Mikhail M Savitski1, Gerard Drewes1.   

Abstract

We extended thermal proteome profiling to detect transmembrane protein-small molecule interactions in cultured human cells. When we assessed the effects of detergents on ATP-binding profiles, we observed shifts in denaturation temperature for ATP-binding transmembrane proteins. We also observed cellular thermal shifts in pervanadate-induced T cell-receptor signaling, delineating the membrane target CD45 and components of the downstream pathway, and with drugs affecting the transmembrane transporters ATP1A1 and MDR1.

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Year:  2015        PMID： 26524241     DOI： 10.1038/nmeth.3652

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  27 in total

1.  Delayed fragmentation and optimized isolation width settings for improvement of protein identification and accuracy of isobaric mass tag quantification on Orbitrap-type mass spectrometers.

Authors:  Mikhail M Savitski; Gavain Sweetman; Manor Askenazi; Jarrod A Marto; Manja Lang; Nico Zinn; Marcus Bantscheff
Journal:  Anal Chem       Date:  2011-11-04       Impact factor: 6.986

Review 2.  How many drug targets are there?

Authors:  John P Overington; Bissan Al-Lazikani; Andrew L Hopkins
Journal:  Nat Rev Drug Discov       Date:  2006-12       Impact factor: 84.694

3.  Pervanadate stabilizes desmosomes.

Authors:  David R Garrod; Christal Fisher; Angela Smith; Zhuxiang Nie
Journal:  Cell Adh Migr       Date:  2008-07-03       Impact factor: 3.405

4.  Measuring and managing ratio compression for accurate iTRAQ/TMT quantification.

Authors:  Mikhail M Savitski; Toby Mathieson; Nico Zinn; Gavain Sweetman; Carola Doce; Isabelle Becher; Fiona Pachl; Bernhard Kuster; Marcus Bantscheff
Journal:  J Proteome Res       Date:  2013-07-02       Impact factor: 4.466

5.  Monitoring drug target engagement in cells and tissues using the cellular thermal shift assay.

Authors:  Daniel Martinez Molina; Rozbeh Jafari; Marina Ignatushchenko; Takahiro Seki; E Andreas Larsson; Chen Dan; Lekshmy Sreekumar; Yihai Cao; Pär Nordlund
Journal:  Science       Date:  2013-07-05       Impact factor: 47.728

6.  Protter: interactive protein feature visualization and integration with experimental proteomic data.

Authors:  Ulrich Omasits; Christian H Ahrens; Sebastian Müller; Bernd Wollscheid
Journal:  Bioinformatics       Date:  2013-10-24       Impact factor: 6.937

7.  The cellular thermal shift assay for evaluating drug target interactions in cells.

Authors:  Rozbeh Jafari; Helena Almqvist; Hanna Axelsson; Marina Ignatushchenko; Thomas Lundbäck; Pär Nordlund; Daniel Martinez Molina
Journal:  Nat Protoc       Date:  2014-08-07       Impact factor: 13.491

8.  Ion coalescence of neutron encoded TMT 10-plex reporter ions.

Authors:  Thilo Werner; Gavain Sweetman; Maria Fälth Savitski; Toby Mathieson; Marcus Bantscheff; Mikhail M Savitski
Journal:  Anal Chem       Date:  2014-03-11       Impact factor: 6.986

9.  Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes.

Authors:  Marcus Bantscheff; Carsten Hopf; Mikhail M Savitski; Antje Dittmann; Paola Grandi; Anne-Marie Michon; Judith Schlegl; Yann Abraham; Isabelle Becher; Giovanna Bergamini; Markus Boesche; Manja Delling; Birgit Dümpelfeld; Dirk Eberhard; Carola Huthmacher; Toby Mathieson; Daniel Poeckel; Valérie Reader; Katja Strunk; Gavain Sweetman; Ulrich Kruse; Gitte Neubauer; Nigel G Ramsden; Gerard Drewes
Journal:  Nat Biotechnol       Date:  2011-01-23       Impact factor: 54.908

10.  Large-scale proteomic analysis of tyrosine-phosphorylation induced by T-cell receptor or B-cell receptor activation reveals new signaling pathways.

Authors:  Masaki Matsumoto; Koji Oyamada; Hidehisa Takahashi; Takamichi Sato; Shigetsugu Hatakeyama; Keiichi I Nakayama
Journal:  Proteomics       Date:  2009-07       Impact factor: 3.984
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  65 in total

1.  Hitting the target.

Authors:  Yves Leestemaker; Huib Ovaa
Journal:  Nat Methods       Date:  2015-12       Impact factor: 28.547

Review 2.  Advances in exploring the therapeutic potential of marine natural products.

Authors:  Xiao Liang; Danmeng Luo; Hendrik Luesch
Journal:  Pharmacol Res       Date:  2019-07-25       Impact factor: 7.658

3.  Impact of Mass Spectrometry-Based Technologies and Strategies on Chemoproteomics as a Tool for Drug Discovery.

Authors:  Ryan A McClure; Jon D Williams
Journal:  ACS Med Chem Lett       Date:  2018-07-03       Impact factor: 4.345

Review 4.  Biophysics in drug discovery: impact, challenges and opportunities.

Authors:  Jean-Paul Renaud; Chun-Wa Chung; U Helena Danielson; Ursula Egner; Michael Hennig; Roderick E Hubbard; Herbert Nar
Journal:  Nat Rev Drug Discov       Date:  2016-08-12       Impact factor: 84.694

5.  Thermal profiling reveals phenylalanine hydroxylase as an off-target of panobinostat.

Authors:  Isabelle Becher; Thilo Werner; Carola Doce; Esther A Zaal; Ina Tögel; Crystal A Khan; Anne Rueger; Marcel Muelbaier; Elsa Salzer; Celia R Berkers; Paul F Fitzpatrick; Marcus Bantscheff; Mikhail M Savitski
Journal:  Nat Chem Biol       Date:  2016-09-26       Impact factor: 15.040

6.  Mutant thermal proteome profiling for characterization of missense protein variants and their associated phenotypes within the proteome.

Authors:  Sarah A Peck Justice; Monica P Barron; Guihong D Qi; H R Sagara Wijeratne; José F Victorino; Ed R Simpson; Jonah Z Vilseck; Aruna B Wijeratne; Amber L Mosley
Journal:  J Biol Chem       Date:  2020-09-02       Impact factor: 5.157

Review 7.  Non-kinase targets of protein kinase inhibitors.

Authors:  Lenka Munoz
Journal:  Nat Rev Drug Discov       Date:  2017-03-10       Impact factor: 84.694

8.  Meltome atlas-thermal proteome stability across the tree of life.

Authors:  Anna Jarzab; Nils Kurzawa; Thomas Hopf; Matthias Moerch; Jana Zecha; Niels Leijten; Yangyang Bian; Eva Musiol; Melanie Maschberger; Gabriele Stoehr; Isabelle Becher; Charlotte Daly; Patroklos Samaras; Julia Mergner; Britta Spanier; Angel Angelov; Thilo Werner; Marcus Bantscheff; Mathias Wilhelm; Martin Klingenspor; Simone Lemeer; Wolfgang Liebl; Hannes Hahne; Mikhail M Savitski; Bernhard Kuster
Journal:  Nat Methods       Date:  2020-04-13       Impact factor: 28.547

9.  ABCG2 requires a single aromatic amino acid to "clamp" substrates and inhibitors into the binding pocket.

Authors:  Tomoka Gose; Talha Shafi; Yu Fukuda; Sourav Das; Yao Wang; Alice Allcock; Ailsa Gavan McHarg; John Lynch; Taosheng Chen; Ikumi Tamai; Anang Shelat; Robert C Ford; John D Schuetz
Journal:  FASEB J       Date:  2020-02-17       Impact factor: 5.191

10.  Genome-Scale Architecture of Small Molecule Regulatory Networks and the Fundamental Trade-Off between Regulation and Enzymatic Activity.

Authors:  Ed Reznik; Dimitris Christodoulou; Joshua E Goldford; Emma Briars; Uwe Sauer; Daniel Segrè; Elad Noor
Journal:  Cell Rep       Date:  2017-09-12       Impact factor: 9.423
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