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Literature DB >> 26499242

Expanding the scope of alkyne-mediated bioconjugations utilizing unnatural amino acids.

Johnathan C Maza¹, Zachary M Nimmo¹, Douglas D Young¹.

Abstract

The importance of bioconjugates within the field of chemistry drives the need for novel methodologies for their preparation. Well-defined and stable bioconjugates are easily accessible via the utilization of unnatural amino acids (UAAs). As such, we have synthesized and incorporated two new UAAs into green fluorescent protein, and optimized a novel Cadiot-Chodkiewicz bioconjugation, effectively expanding the toolbox of chemical reactions that can be employed in the preparation of bioconjugates.

Entities: CellLine Chemical Disease Species

Mesh：

Substances：

Year: 2016 PMID： 26499242 PMCID： PMC5461652 DOI： 10.1039/c5cc08287k

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

31 in total

Review 1. Adding new chemistries to the genetic code.

Authors: Chang C Liu; Peter G Schultz
Journal: Annu Rev Biochem Date: 2010 Impact factor: 23.643

2. Cadiot-Chodkiewicz active template synthesis of rotaxanes and switchable molecular shuttles with weak intercomponent interactions.

Authors: José Berná; Stephen M Goldup; Ai-Lan Lee; David A Leigh; Mark D Symes; Gilberto Teobaldi; Francesco Zerbetto
Journal: Angew Chem Int Ed Engl Date: 2008 Impact factor: 15.336

Review 3. Advances in enzyme immobilisation.

Authors: Dean Brady; Justin Jordaan
Journal: Biotechnol Lett Date: 2009-07-10 Impact factor: 2.461

Review 5. Playing with the Molecules of Life.

Authors: Douglas D Young; Peter G Schultz
Journal: ACS Chem Biol Date: 2018-03-02 Impact factor: 5.100

5 in total

Expanding the scope of alkyne-mediated bioconjugations utilizing unnatural amino acids.

Review 1. Adding new chemistries to the genetic code.

2. Cadiot-Chodkiewicz active template synthesis of rotaxanes and switchable molecular shuttles with weak intercomponent interactions.

Review 3. Advances in enzyme immobilisation.

4. Site-specific protein immobilization using unnatural amino acids.

5. Synthetase polyspecificity as a tool to modulate protein function.

Review 6. Synthesis of naturally occurring polyynes.

Review 7. Cu-free click cycloaddition reactions in chemical biology.

8. Presentation and detection of azide functionality in bacterial cell surface proteins.

9. Bioconjugation by copper(I)-catalyzed azide-alkyne [3 + 2] cycloaddition.

10. A strain-promoted [3 + 2] azide-alkyne cycloaddition for covalent modification of biomolecules in living systems.

1. Utilization of alkyne bioconjugations to modulate protein function.

2. Synthesis and incorporation of a caged tyrosine amino acid possessing a bioorthogonal handle.

3. Development of optimized conditions for Glaser-Hay bioconjugations.

4. Mechanistic investigation and further optimization of the aqueous Glaser-Hay bioconjugation.

Review 5. Playing with the Molecules of Life.