Literature DB >> 26447562

Measurement of Postreplicative DNA Metabolism and Damage in the Rodent Brain.

Jay P Patel1, Mark L Sowers1, Jason L Herring1, Jacob A Theruvathu1, Mark R Emmett1, Bridget E Hawkins1, Kangling Zhang1, Douglas S DeWitt1, Donald S Prough1, Lawrence C Sowers1.   

Abstract

The DNA of all organisms is metabolically active due to persistent endogenous DNA damage, repair, and enzyme-mediated base modification pathways important for epigenetic reprogramming and antibody diversity. The free bases released from DNA either spontaneously or by base excision repair pathways constitute DNA metabolites in living tissues. In this study, we have synthesized and characterized the stable-isotope standards for a series of pyrimidines derived from the normal DNA bases by oxidation and deamination. We have used these standards to measure free bases in small molecule extracts from rat brain. Free bases are observed in extracts, consistent with both endogenous DNA damage and 5-methylcytosine demethylation pathways. The most abundant free base observed is uracil, and the potential sources of uracil are discussed. The free bases measured in tissue extracts constitute the end product of DNA metabolism and could be used to reveal metabolic disturbances in human disease.

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Year:  2015        PMID： 26447562      PMCID： PMC7986959          DOI： 10.1021/acs.chemrestox.5b00359

Source DB:  PubMed          Journal:  Chem Res Toxicol        ISSN： 0893-228X            Impact factor:   3.739


  59 in total

1.  Hydroxylation of 5-methylcytosine by TET1 promotes active DNA demethylation in the adult brain.

Authors:  Junjie U Guo; Yijing Su; Chun Zhong; Guo-li Ming; Hongjun Song
Journal:  Cell       Date:  2011-04-14       Impact factor: 41.582

Review 2.  Active DNA demethylation: many roads lead to Rome.

Authors:  Susan C Wu; Yi Zhang
Journal:  Nat Rev Mol Cell Biol       Date:  2010-08-04       Impact factor: 94.444

3.  Thymine DNA glycosylase can rapidly excise 5-formylcytosine and 5-carboxylcytosine: potential implications for active demethylation of CpG sites.

Authors:  Atanu Maiti; Alexander C Drohat
Journal:  J Biol Chem       Date:  2011-08-23       Impact factor: 5.157

Review 4.  Cytidine deaminases: AIDing DNA demethylation?

Authors:  Eric L Fritz; F Nina Papavasiliou
Journal:  Genes Dev       Date:  2010-10-01       Impact factor: 11.361

5.  Synthesis of stable-isotope enriched 5-methylpyrimidines and their use as probes of base reactivity in DNA.

Authors:  Artur Burdzy; Katherine T Noyes; Victoria Valinluck; Lawrence C Sowers
Journal:  Nucleic Acids Res       Date:  2002-09-15       Impact factor: 16.971

6.  Enzymatic methylation of DNA in cultured human cells studied by stable isotope incorporation and mass spectrometry.

Authors:  Jason L Herring; Daniel K Rogstad; Lawrence C Sowers
Journal:  Chem Res Toxicol       Date:  2009-06       Impact factor: 3.739

7.  Novel modification of 5-formyluracil by cysteine derivatives in aqueous solution.

Authors:  H Terato; H Morita; Y Ohyama; H Ide
Journal:  Nucleosides Nucleotides       Date:  1998 Jan-Mar

8.  Phosphorolysis of (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) and other 5-substituted-2'-deoxyuridines by purified human thymidine phosphorylase and intact blood platelets.

Authors:  C Desgranges; G Razaka; M Rabaud; H Bricaud; J Balzarini; E De Clercq
Journal:  Biochem Pharmacol       Date:  1983-12-01       Impact factor: 5.858

9.  5-Hydroxymethylcytosine is a predominantly stable DNA modification.

Authors:  Martin Bachman; Santiago Uribe-Lewis; Xiaoping Yang; Michael Williams; Adele Murrell; Shankar Balasubramanian
Journal:  Nat Chem       Date:  2014-09-21       Impact factor: 24.427

10.  Role of Tet proteins in 5mC to 5hmC conversion, ES-cell self-renewal and inner cell mass specification.

Authors:  Shinsuke Ito; Ana C D'Alessio; Olena V Taranova; Kwonho Hong; Lawrence C Sowers; Yi Zhang
Journal:  Nature       Date:  2010-08-26       Impact factor: 49.962
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  3 in total

Review 1.  Formation and repair of oxidatively generated damage in cellular DNA.

Authors:  Jean Cadet; Kelvin J A Davies; Marisa Hg Medeiros; Paolo Di Mascio; J Richard Wagner
Journal:  Free Radic Biol Med       Date:  2017-01-02       Impact factor: 7.376

2.  Chemical and enzymatic modifications of 5-methylcytosine at the intersection of DNA damage, repair, and epigenetic reprogramming.

Authors:  Tuvshintugs Baljinnyam; Mark L Sowers; Chia Wei Hsu; James W Conrad; Jason L Herring; Linda C Hackfeld; Lawrence C Sowers
Journal:  PLoS One       Date:  2022-08-29       Impact factor: 3.752

Review 3.  Spectroscopic and in vitro Investigations of Fe2+ /α-Ketoglutarate-Dependent Enzymes Involved in Nucleic Acid Repair and Modification.

Authors:  David Schmidl; Niko S W Lindlar Né Jonasson; Annika Menke; Sabine Schneider; Lena J Daumann
Journal:  Chembiochem       Date:  2022-02-15       Impact factor: 3.461
  3 in total

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