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Literature DB >> 26144228

Use of dynamic light scattering and small-angle X-ray scattering to characterize new surfactants in solution conditions for membrane-protein crystallization.

Mohamed Dahani¹, Laurie Anne Barret¹, Simon Raynal¹, Colette Jungas², Pétra Pernot³, Ange Polidori¹, Françoise Bonneté¹.

Abstract

The structural and interactive properties of two novel hemifluorinated surfactants, F2H9-β-M and F4H5-β-M, the syntheses of which were based on the structure and hydrophobicity of the well known dodecyl-β-maltoside (DD-β-M), are described. The shape of their micellar assemblies was characterized by small-angle X-ray scattering and their intermicellar interactions in crystallizing conditions were measured by dynamic light scattering. Such information is essential for surfactant phase-diagram determination and membrane-protein crystallization.

Entities: Chemical

Keywords: crystallization; membrane protein; phase diagram; scattering techniques; surfactant micelle

Mesh：

Substances：

Year: 2015 PMID： 26144228 PMCID： PMC4498704 DOI： 10.1107/S2053230X15009516

Source DB: PubMed Journal: Acta Crystallogr F Struct Biol Commun ISSN： 2053-230X Impact factor: 1.056

31 in total

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Authors: Pil Seok Chae; Kamil Gotfryd; Jennifer Pacyna; Larry J W Miercke; Søren G F Rasmussen; Rebecca A Robbins; Rohini R Rana; Claus J Loland; Brian Kobilka; Robert Stroud; Bernadette Byrne; Ulrik Gether; Samuel H Gellman
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Authors: Shaoxin Li; Da Xing; Junfeng Li
Journal: J Biol Phys Date: 2004-01 Impact factor: 1.365

7. Influence of hydrophobic micelle structure on crystallization of the photosynthetic RC-LH1-PufX complex from Rhodobacter blasticus.

Authors: Laurie-Anne Barret; Cherone Barrot-Ivolot; Simon Raynal; Colette Jungas; Ange Polidori; Françoise Bonneté
Journal: J Phys Chem B Date: 2013-07-10 Impact factor: 2.991

8. A new class of amphiphiles bearing rigid hydrophobic groups for solubilization and stabilization of membrane proteins.

Authors: Pil Seok Chae; Søren G F Rasmussen; Rohini R Rana; Kamil Gotfryd; Andrew C Kruse; Aashish Manglik; Kyung Ho Cho; Shailika Nurva; Ulrik Gether; Lan Guan; Claus J Loland; Bernadette Byrne; Brian K Kobilka; Samuel H Gellman
Journal: Chemistry Date: 2012-06-22 Impact factor: 5.236