Draft genome sequences of eight type strains of the genus demequina.

Here, we report the draft genome sequences of the type strains of Demequina aestuarii, Demequina aurantiaca, Demequina flava, Demequina globuliformis, Demequina lutea, Demequina oxidasica, Demequina salsinemoris, and Demequina sediminicola. The genome sequences presented here will facilitate taxonomical, ecological, and functional studies of members of the genus Demequina.

GENOME ANNOUNCEMENT

The genus Demequina, which belongs to the family Demequinaceae within the suborder Micrococcineae, is composed of Gram stain-positive, rod-shaped, and non-endospore-forming actinobacteria (1, 2). At the time of writing, this genus contains 8 species with validly published names: Demequina aestuarii (1), Demequina aurantiaca, Demequina globuliformis, Demequina oxidasica (2), Demequina flava, Demequina sediminicola (3), Demequina lutea (4), and Demequina salsinemoris (5). So far, members of the genus were mainly isolated from samples of marine origin, such as tidal flat and sea sediments. The genus Demequina is characterized by the presence of a discriminative menaquinone, namely, demethylmenaquinone DMK-9(H4). Furthermore, the peptidoglycan type of this genus is of A4β type with ornithine as the diagnostic diamino acid (6), and this feature distinguishes the genus Demequina from the closely related genus Lysinimicrobium within the family Demequinaceae (7). To reveal genomic features of the genus Demequina, we performed whole-genome shotgun sequencing of all type strains of the genus.

Genomic DNAs of D. aestuarii NBRC 106260T, D. aurantiaca NBRC 106265T, D. flava NBRC 105854T, D. globuliformis NBRC 106266T, D. lutea NBRC 106155T, D. oxidasica NBRC 106264T, D. salsinemoris NBRC 105323T, and D. sediminicola NBRC 105855T were extracted and purified from liquid-dried cells in ampules provided from the NBRC culture collection using the EZ1 DNA tissue kit and EZ1 advanced instruments (Qiagen). The whole genomes of these strains were analyzed by using paired-end sequencing with MiSeq (Illumina). These reads were assembled using the Newbler v2.6 software and subsequently finished using the GenoFinisher software (8). The results of the sequencing are summarized in Table 1.

TABLE 1

Summary of genome sequencing in the present study

Organism	Read (Mb)	Fold coverage	No. of scaffolds	Genome size (bp)	G+C content (%)	Accession no.
D. aestuarii NBRC 106260T	291	104	3	2,790,392	69.1	BBRD00000000
D. aurantiaca NBRC 106265T	265	103	4	2,565,694	64.6	BBRF00000000
D. flava NBRC 105854T	215	84	3	2,546,965	64.7	BBRA00000000
D. globuliformis NBRC 106266T	262	100	7	2,622,354	66.3	BBRG00000000
D. lutea NBRC 106155T	264	100	40	2,643,741	65.9	BBRC00000000
D. oxidasica NBRC 106264T	224	85	5	2,623,557	64.1	BBRE00000000
D. salsinemoris NBRC 105323T	215	67	14	3,209,522	70.2	BBQZ00000000
D. sediminicola NBRC 105855T	226	92	8	2,460,989	62.7	BBRB00000000

The genome sequences reported in this study will provide a foundation for further phylogenetic, taxonomic, comparative genomic, metagenomic, and functional studies of the genus Demequina and related taxa. Recently, we isolated some novel strains which are closely related to the genus Demequina and will perform a taxonomic study for the classification of the isolated strains. In the process of the study, the genome sequences presented here will facilitate phylogenetic analyses, including in silico DNA-DNA hybridization. Detailed reports of these analyses will be included in a future publication.

Nucleotide sequence accession numbers.

The draft genome sequences of D. aestuarii NBRC 106260T, D. aurantiaca NBRC 106265T, D. flava NBRC 105854T, D. globuliformis NBRC 106266T, D. lutea NBRC 106155T, D. oxidasica NBRC 106264T, D. salsinemoris NBRC 105323T, and D. sediminicola NBRC 105855T have been deposited in DDBJ/ENA/GenBank. The accession numbers are listed in Table 1. The versions described in this paper are the first versions.