| Literature DB >> 25799440 |
Jeffrey C Miller1, Lei Zhang1, Danny F Xia1, John J Campo1, Irina V Ankoudinova1, Dmitry Y Guschin1, Joshua E Babiarz1, Xiangdong Meng1, Sarah J Hinkley1, Stephen C Lam1, David E Paschon1, Anna I Vincent1, Gladys P Dulay1, Kyle A Barlow1, David A Shivak1, Elo Leung1, Jinwon D Kim1, Rainier Amora1, Fyodor D Urnov1, Philip D Gregory1, Edward J Rebar1.
Abstract
Transcription activator-like effector (TALE) proteins have gained broad appeal as a platform for targeted DNA recognition, largely owing to their simple rules for design. These rules relate the base specified by a single TALE repeat to the identity of two key residues (the repeat variable diresidue, or RVD) and enable design for new sequence targets via modular shuffling of these units. A key limitation of these rules is that their simplicity precludes options for improving designs that are insufficiently active or specific. Here we address this limitation by developing an expanded set of RVDs and applying them to improve the performance of previously described TALEs. As an extreme example, total conversion of a TALE nuclease to new RVDs substantially reduced off-target cleavage in cellular studies. By providing new RVDs and design strategies, these studies establish options for developing improved TALEs for broader application across medicine and biotechnology.Entities:
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Year: 2015 PMID： 25799440 DOI： 10.1038/nmeth.3330
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547