OBJECTIVES/HYPOTHESIS: To demonstrate the dosimetry effect of electromechanical reshaping (EMR) on cartilage shape change, structural integrity, cellular viability, and remodeling of grafts in an in vivo long-term animal model. STUDY DESIGN: Animal study. METHODS: A subperichondrial cartilaginous defect was created within the base of the pinna of 31 New Zealand white rabbits. Autologous costal cartilage grafts were electromechanically reshaped to resemble the rabbit auricular base framework and mechanically secured into the pinna base defect. Forty-nine costal cartilage specimens (four control and 45 experimental) successfully underwent EMR using a paired set of voltage-time combinations and survived for 6 or 12 weeks. Shape change was measured, and specimens were analyzed using digital imaging, tissue histology, and confocal microscopy with LIVE-DEAD viability assays. RESULTS: Shape change was proportional to charge transfer in all experimental specimens (P < .01) and increased with voltage. All experimental specimens contoured to the auricular base. Focal cartilage degeneration and fibrosis was observed where needle electrodes were inserted, ranging from 2.2 to 3.9 mm. The response to injury increased with increasing charge transfer and survival duration. CONCLUSIONS: EMR results in appropriate shape change in cartilage grafts with chondrocyte injury highly localized. These studies suggest that elements of auricular reconstruction may be feasible using EMR. Extended survival periods and further optimization of voltage-time pairs are necessary to evaluate the long-term effects and shape-change potential of EMR. LEVELS OF EVIDENCE: NA.
OBJECTIVES/HYPOTHESIS: To demonstrate the dosimetry effect of electromechanical reshaping (EMR) on cartilage shape change, structural integrity, cellular viability, and remodeling of grafts in an in vivo long-term animal model. STUDY DESIGN: Animal study. METHODS: A subperichondrial cartilaginous defect was created within the base of the pinna of 31 New Zealand white rabbits. Autologous costal cartilage grafts were electromechanically reshaped to resemble the rabbit auricular base framework and mechanically secured into the pinna base defect. Forty-nine costal cartilage specimens (four control and 45 experimental) successfully underwent EMR using a paired set of voltage-time combinations and survived for 6 or 12 weeks. Shape change was measured, and specimens were analyzed using digital imaging, tissue histology, and confocal microscopy with LIVE-DEAD viability assays. RESULTS: Shape change was proportional to charge transfer in all experimental specimens (P < .01) and increased with voltage. All experimental specimens contoured to the auricular base. Focal cartilage degeneration and fibrosis was observed where needle electrodes were inserted, ranging from 2.2 to 3.9 mm. The response to injury increased with increasing charge transfer and survival duration. CONCLUSIONS: EMR results in appropriate shape change in cartilage grafts with chondrocyte injury highly localized. These studies suggest that elements of auricular reconstruction may be feasible using EMR. Extended survival periods and further optimization of voltage-time pairs are necessary to evaluate the long-term effects and shape-change potential of EMR. LEVELS OF EVIDENCE: NA.
Authors: Dongrim Seol; Daniel J McCabe; Hyeonghun Choe; Hongjun Zheng; Yin Yu; Keewoong Jang; Morgan W Walter; Abigail D Lehman; Lei Ding; Joseph A Buckwalter; James A Martin Journal: Arthritis Rheum Date: 2012-11
Authors: Edward C Kuan; Ashley A Hamamoto; Cyrus T Manuel; Dmitriy E Protsenko; Brian J F Wong Journal: Laryngoscope Date: 2014-05-02 Impact factor: 3.325
Authors: Edward C Wu; Dmitriy E Protsenko; Adam Z Khan; Sterling Dubin; Koohyar Karimi; Brian J F Wong Journal: IEEE Trans Biomed Eng Date: 2011-05-19 Impact factor: 4.538
Authors: Cyrus T Manuel; Allen Foulad; Dmitriy E Protsenko; Ashley Hamamoto; Brian J F Wong Journal: Laryngoscope Date: 2011-08-16 Impact factor: 3.325
Authors: Ki-Hong Kevin Ho; Sergio H Diaz Valdes; Dmitriy E Protsenko; Guillermo Aguilar; Brian J F Wong Journal: Laryngoscope Date: 2003-11 Impact factor: 3.325
Authors: Sepehr Oliaei; Cyrus Manuel; Badran Karam; Syed F Hussain; Ashley Hamamoto; Dmitriy E Protsenko; Brian J F Wong Journal: JAMA Facial Plast Surg Date: 2013-01 Impact factor: 4.611
Authors: Andrew E Heidari; Ellen M Hong; Asher Park; Tiffany T Pham; Earl Steward; Lily Y Chen; Yueqiao Qu; Brandyn S Dunn; Soo H Seo; Urja Patel; Katelyn Dilley; Amir A Hakimi; Adeela Syed; Sehwan Kim; Michael G Hill; Joon S You; Brian J F Wong Journal: Lasers Surg Med Date: 2021-08-19 Impact factor: 4.025
Authors: Dana M Hutchison; Amir A Hakimi; Avin Wijayaweera; Soohong Seo; Ellen M Hong; Tiffany T Pham; Melissa Bircan; Ryan Sivoraphonh; Brandyn Dunn; Mark R Kobayashi; Sehwan Kim; Brian Jf Wong Journal: Scars Burn Heal Date: 2021-03-16
Authors: Katelyn K Dilley; Pamela A Borden; Yueqiao Qu; Andrew E Heidari; Karthik R Prasad; Yan Li; Chung Ho Sun; Zhongping Chen; Sehwan Kim; Michael G Hill; Brian J F Wong Journal: Transl Vis Sci Technol Date: 2022-01-03 Impact factor: 3.283