| Literature DB >> 25669616 |
Gijs van der Schot1, Martin Svenda1, Filipe R N C Maia2, Max Hantke1, Daniel P DePonte3, M Marvin Seibert4, Andrew Aquila5, Joachim Schulz5, Richard Kirian6, Mengning Liang6, Francesco Stellato7, Bianca Iwan1, Jakob Andreasson1, Nicusor Timneanu1, Daniel Westphal1, F Nunes Almeida1, Dusko Odic1, Dirk Hasse1, Gunilla H Carlsson1, Daniel S D Larsson1, Anton Barty8, Andrew V Martin9, Sebastian Schorb10, Christoph Bostedt10, John D Bozek10, Daniel Rolles6, Artem Rudenko11, Sascha Epp6, Lutz Foucar12, Benedikt Rudek13, Robert Hartmann14, Nils Kimmel15, Peter Holl14, Lars Englert16, Ne-Te Duane Loh17, Henry N Chapman18, Inger Andersson1, Janos Hajdu19, Tomas Ekeberg1.
Abstract
There exists a conspicuous gap of knowledge about the organization of life at mesoscopic levels. Ultra-fast coherent diffractive imaging with X-ray free-electron lasers can probe structures at the relevant length scales and may reach sub-nanometer resolution on micron-sized living cells. Here we show that we can introduce a beam of aerosolised cyanobacteria into the focus of the Linac Coherent Light Source and record diffraction patterns from individual living cells at very low noise levels and at high hit ratios. We obtain two-dimensional projection images directly from the diffraction patterns, and present the results as synthetic X-ray Nomarski images calculated from the complex-valued reconstructions. We further demonstrate that it is possible to record diffraction data to nanometer resolution on live cells with X-ray lasers. Extension to sub-nanometer resolution is within reach, although improvements in pulse parameters and X-ray area detectors will be necessary to unlock this potential.Mesh：
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Year: 2015 PMID： 25669616 DOI： 10.1038/ncomms6704
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919