OBJECTIVE: To correlate corneal endothelium-Descemet membrane (EDM) layer parameters of scroll tightness with donor age, endothelial cell density (ECD), and history of diabetes. METHODS: Endothelium-Descemet membrane layer scrolls were harvested from 26 corneoscleral buttons using the SCUBA technique by a cornea-fellowship trained ophthalmologist masked to donor age. Two independent outcome parameters were used to characterize the scrolling severity of successfully harvested tissue: scroll width and tendency for EDM scroll formation (referred to as scroll rating on a 1-4 scale: incomplete scroll formation to tightly scrolled). RESULTS: Mean donor age was 59 ± 17 (15-69) years. Mean ECD of EDM scroll was 2,451 ± 626 (range: 1,307-3,195) cells per square millimeter. Using stepwise linear regression, a significant correlation was found between scroll width and donor age (R=0.497, P<0.05). Additionally, a significant inverse correlation was found between scroll width and ECD (R=-0.605, P<0.05). There was no statistically significant correlation between a donor history of diabetes and the parameters of scrolling tendency. CONCLUSIONS: Our data suggest that using older donors reduces EDM scroll tightness.
OBJECTIVE: To correlate corneal endothelium-Descemet membrane (EDM) layer parameters of scroll tightness with donor age, endothelial cell density (ECD), and history of diabetes. METHODS: Endothelium-Descemet membrane layer scrolls were harvested from 26 corneoscleral buttons using the SCUBA technique by a cornea-fellowship trained ophthalmologist masked to donor age. Two independent outcome parameters were used to characterize the scrolling severity of successfully harvested tissue: scroll width and tendency for EDM scroll formation (referred to as scroll rating on a 1-4 scale: incomplete scroll formation to tightly scrolled). RESULTS: Mean donor age was 59 ± 17 (15-69) years. Mean ECD of EDM scroll was 2,451 ± 626 (range: 1,307-3,195) cells per square millimeter. Using stepwise linear regression, a significant correlation was found between scroll width and donor age (R=0.497, P<0.05). Additionally, a significant inverse correlation was found between scroll width and ECD (R=-0.605, P<0.05). There was no statistically significant correlation between a donor history of diabetes and the parameters of scrolling tendency. CONCLUSIONS: Our data suggest that using older donors reduces EDM scroll tightness.
Authors: Sonja Heinzelmann; Silja Hüther; Daniel Böhringer; Philipp Eberwein; Thomas Reinhard; Philip Maier Journal: Cornea Date: 2014-06 Impact factor: 2.651
Authors: Frederico P Guerra; Arundhati Anshu; Marianne O Price; Arthur W Giebel; Francis W Price Journal: Ophthalmology Date: 2011-08-27 Impact factor: 12.079
Authors: Friedrich E Kruse; Kathrin Laaser; Claus Cursiefen; Ludwig M Heindl; Ursula Schlötzer-Schrehardt; Stephan Riss; Björn O Bachmann Journal: Cornea Date: 2011-05 Impact factor: 2.651