Jun Hu1, Yi Li2, Lin Chen3, Zhengrong Yang3, Guanglu Zhao4, Yushu Wang2, Jinquan Cheng3, Jin Zhao5, Ying Peng6. 1. Department of Life Sciences, Sun Yat-Sen University, Guangzhou, China; Shenzhen Center for Disease Control and Prevention, Shenzhen, China; Department of Science in Botany, Jilin University, Changchun, China. 2. Department of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China. 3. Shenzhen Center for Disease Control and Prevention, Shenzhen, China. 4. Shenzhen Center for Chronic Disease Control, Shenzhen, China. 5. Shenzhen Center for Disease Control and Prevention, Shenzhen, China. Electronic address: szhaojin@gmail.com. 6. Department of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China. Electronic address: 2353352460@qq.com.
Abstract
BACKGROUND/ PURPOSE: To analyze the polymorphism of the IL-22 gene in Han Chinese patients and to evaluate the influence of IL-22 polymorphism on human immunodeficiency virus (HIV) infection. METHODS: IL-22 gene polymorphism was analyzed in 73 blood samples from healthy participants. The influence of the genotype and allele distribution of three single nucleotide polymorphisms (rs2227484, rs2227485, and rs2227513) of IL-22 on HIV infection was evaluated in 619 HIV seropositive patients and 619 healthy controls. To determine the association between the rs2227513 genotype and IL-22 levels in plasma, we randomly selected 29 HIV seropositive blood samples and 15 healthy blood samples and measured the levels of IL-22. RESULTS: Nine single nucleotide polymorphism loci of the IL-22 gene were found (rs2227484, rs2227485, rs2227491, rs2227508, rs2227513, rs1179249, rs1179250, rs1179251, and rs1182844). Stratified analysis (by sex) showed a higher association of HIV infection and the A/G genotype and G allele at rs2227513 in women, but not in men (A/G genotype odds ratio = 5.24, 95% confidence interval = 1.13-24.27; allele G odds ratio = 5.27, 95% confidence interval 1.15-24.23). The rs2227513 A/G genotype was also associated with significantly higher levels of plasma IL-22, regardless of whether the patient was HIV seropositive or seronegative. CONCLUSION: Our results suggest that IL-22 production in blood might act as a pathogenic factor in HIV infection.
BACKGROUND/ PURPOSE: To analyze the polymorphism of the IL-22 gene in Han Chinese patients and to evaluate the influence of IL-22 polymorphism on human immunodeficiency virus (HIV) infection. METHODS:IL-22 gene polymorphism was analyzed in 73 blood samples from healthy participants. The influence of the genotype and allele distribution of three single nucleotide polymorphisms (rs2227484, rs2227485, and rs2227513) of IL-22 on HIV infection was evaluated in 619 HIV seropositivepatients and 619 healthy controls. To determine the association between the rs2227513 genotype and IL-22 levels in plasma, we randomly selected 29 HIV seropositive blood samples and 15 healthy blood samples and measured the levels of IL-22. RESULTS: Nine single nucleotide polymorphism loci of the IL-22 gene were found (rs2227484, rs2227485, rs2227491, rs2227508, rs2227513, rs1179249, rs1179250, rs1179251, and rs1182844). Stratified analysis (by sex) showed a higher association of HIV infection and the A/G genotype and G allele at rs2227513 in women, but not in men (A/G genotype odds ratio = 5.24, 95% confidence interval = 1.13-24.27; allele G odds ratio = 5.27, 95% confidence interval 1.15-24.23). The rs2227513 A/G genotype was also associated with significantly higher levels of plasma IL-22, regardless of whether the patient was HIV seropositive or seronegative. CONCLUSION: Our results suggest that IL-22 production in blood might act as a pathogenic factor in HIV infection.
Authors: Nada H Aljarba; Mashael R Al-Anazi; Mohammed I Shafeai; Fuad H Rudiny; Saad M Bin Dajem; Hani Alothaid; Majid Darraj; Saad Alkahtani; Jahad Alghamdi; Mohammed N Al-Ahdal; Ahmed A Al-Qahtani Journal: Mediators Inflamm Date: 2020-02-19 Impact factor: 4.711