Zohreh Borzooy1, Adrian Streinu-Cercel2, Abbass Mirshafiey3, Azam Khamseh4, Masoud Karkhaneh Mahmoudie4, Shadi Sadat Navabi5, Marjan Nosrati6, Zahra Najafi7, Mostafa Hosseini8, Seyed Mohammad Jazayeri9. 1. PhD student, Department of Infectious Diseases, Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania; Department of Immunology and Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 2. MD, PhD, Department of Infectious Diseases, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania; National Institute of Infectious Diseases, "Prof. Dr. Matei Balş", Bucharest, Romania. 3. Ms, PhD, Head of the department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 4. Bs, Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 5. Ms, Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran. 6. Bs, Department of Infection Control, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran. 7. Ms, Bahrami Hospital, Tehran University of Medical Sciences, Tehran, Iran. 8. Ms, PhD, Department of Epidemiology and Biostatistics School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 9. MD, PhD, Clinical Virologist, Hepatitis B Molecular Laboratory, Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND: Healthcare workers constitute a population at high risk for HBV infection. Efficient vaccination options are available; however, the individual response to HBV vaccination may vary widely between subjects, potentially due to cytokine profiles and genetic variations. In the present study, we investigated the relationship between IL-17 and IL-22 gene polymorphisms versus non- and low-responsiveness to HBV vaccination in healthcare workers. METHODS: We selected the following IL-17 and IL-22 polymorphisms: rs4711998 (A/G) from IL-17 and rs2227501 (A/T), rs2227503 (A/G), rs1026786 (A/G) from IL-22 sequences genes. These were determined by polymerase chain reaction restriction fragment length polymorphisms. RESULTS: The IL-17 rs4711998 GG genotype had a significantly lower frequency in non-responders compared to low-responders (p=0.025). However, we did not identify a relationship between IL-22 rs1026780, rs2227501 and rs2227503 genotypes and the anti-HBs response following HBV vaccination. CONCLUSION: These data suggest that genetic variation in rs4711998 polymorphisms in the IL-17 cytokine may influence vaccine-induced immune responses to HBV vaccine in healthcare workers.
BACKGROUND: Healthcare workers constitute a population at high risk for HBV infection. Efficient vaccination options are available; however, the individual response to HBV vaccination may vary widely between subjects, potentially due to cytokine profiles and genetic variations. In the present study, we investigated the relationship between IL-17 and IL-22 gene polymorphisms versus non- and low-responsiveness to HBV vaccination in healthcare workers. METHODS: We selected the following IL-17 and IL-22 polymorphisms: rs4711998 (A/G) from IL-17 and rs2227501 (A/T), rs2227503 (A/G), rs1026786 (A/G) from IL-22 sequences genes. These were determined by polymerase chain reaction restriction fragment length polymorphisms. RESULTS: The IL-17rs4711998 GG genotype had a significantly lower frequency in non-responders compared to low-responders (p=0.025). However, we did not identify a relationship between IL-22rs1026780, rs2227501 and rs2227503 genotypes and the anti-HBs response following HBV vaccination. CONCLUSION: These data suggest that genetic variation in rs4711998 polymorphisms in the IL-17 cytokine may influence vaccine-induced immune responses to HBV vaccine in healthcare workers.
Authors: Ye Zhang; Melissa A Cobleigh; Jian-Qi Lian; Chang-Xing Huang; Carmen J Booth; Xue-Fan Bai; Michael D Robek Journal: Gastroenterology Date: 2011-06-25 Impact factor: 22.682
Authors: Branwen J Hennig; Angela J Frodsham; Simon Hellier; Susanne Knapp; Leland J Yee; Mark Wright; Lyna Zhang; Howard C Thomas; Mark Thursz; Adrian V Hill Journal: Liver Int Date: 2007-10 Impact factor: 5.828