Literature DB >> 25511010

Nanostructured microfluidic digestion system for rapid high-performance proteolysis.

Gong Cheng1, Si-Jie Hao, Xu Yu, Si-Yang Zheng.   

Abstract

A novel microfluidic protein digestion system with a nanostructured and bioactive inner surface was constructed by an easy biomimetic self-assembly strategy for rapid and effective proteolysis in 2 minutes, which is faster than the conventional overnight digestion methods. It is expected that this work would contribute to rapid online digestion in future high-throughput proteomics.

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Mesh：

Year:  2015        PMID： 25511010      PMCID： PMC4304898          DOI： 10.1039/c4lc01165a

Source DB:  PubMed          Journal:  Lab Chip        ISSN： 1473-0189            Impact factor:   6.799


  27 in total

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5.  In-gel digestion for mass spectrometric characterization of proteins and proteomes.

Authors:  Andrej Shevchenko; Henrik Tomas; Jan Havlis; Jesper V Olsen; Matthias Mann
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

6.  Novel microwave-assisted digestion by trypsin-immobilized magnetic nanoparticles for proteomic analysis.

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Journal:  Mol Biosyst       Date:  2011-07-29

Review 8.  Microfluidic spinning of micro- and nano-scale fibers for tissue engineering.

Authors:  Yesl Jun; Edward Kang; Sukyoung Chae; Sang-Hoon Lee
Journal:  Lab Chip       Date:  2014-07-07       Impact factor: 6.799

9.  Full dynamic range proteome analysis of S. cerevisiae by targeted proteomics.

Authors:  Paola Picotti; Bernd Bodenmiller; Lukas N Mueller; Bruno Domon; Ruedi Aebersold
Journal:  Cell       Date:  2009-08-06       Impact factor: 41.582

10.  A multistage volumetric bar chart chip for visualized quantification of DNA.

Authors:  Yujun Song; Yuanchen Wang; Lidong Qin
Journal:  J Am Chem Soc       Date:  2013-10-29       Impact factor: 15.419
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  1 in total

Review 1.  Tumor-on-a-chip for integrating a 3D tumor microenvironment: chemical and mechanical factors.

Authors:  L Wan; C A Neumann; P R LeDuc
Journal:  Lab Chip       Date:  2020-03-03       Impact factor: 6.799
  1 in total

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