| Literature DB >> 17406544 |
Andrej Shevchenko1, Henrik Tomas, Jan Havlis, Jesper V Olsen, Matthias Mann.
Abstract
In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.Entities:
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Year: 2006 PMID： 17406544 DOI： 10.1038/nprot.2006.468
Source DB: PubMed Journal: Nat Protoc ISSN： 1750-2799 Impact factor: 13.491