Literature DB >> 25335641

Metabolic oxygen consumption measurement with a single-cell biosensor after particle microbeam irradiation.

Yanping Xu1, Bo Zhang2, Mark Messerli3,4, Gerhard Randers-Pehrson5, Tom K Hei2, David J Brenner5.   

Abstract

A noninvasive, self-referencing biosensor/probe system has been integrated into the Columbia University Radiological Research Accelerator Facility Microbeam II end station. A single-cell oxygen consumption measurement has been conducted with this type of oxygen probe in 37° C Krebs-Ringer Bicarbonate buffer immediately before and after a single-cell microbeam irradiation. It is the first such measurement made for a microbeam irradiation, and a six fold increment of oxygen flux induced during a 15-s period of time has been observed following radiation exposure. The experimental procedure and the results are discussed.

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Mesh：

Year:  2014        PMID： 25335641      PMCID： PMC4437628          DOI： 10.1007/s00411-014-0574-1

Source DB:  PubMed          Journal:  Radiat Environ Biophys        ISSN： 0301-634X            Impact factor:   1.925


  10 in total

1.  Nerve injury induces a rapid efflux of nitric oxide (NO) detected with a novel NO microsensor.

Authors:  S M Kumar; D M Porterfield; K J Muller; P J Smith; C L Sahley
Journal:  J Neurosci       Date:  2001-01-01       Impact factor: 6.167

2.  Induction of radioresistance by a nitric oxide-mediated bystander effect.

Authors:  H Matsumoto; S Hayashi; M Hatashita; K Ohnishi; H Shioura; T Ohtsubo; R Kitai; T Ohnishi; E Kano
Journal:  Radiat Res       Date:  2001-03       Impact factor: 2.841

3.  An accelerator-based neutron microbeam system for studies of radiation effects.

Authors:  Yanping Xu; Gerhard Randers-Pehrson; Stephen A Marino; Alan W Bigelow; Mark S Akselrod; Jeff G Sykora; David J Brenner
Journal:  Radiat Prot Dosimetry       Date:  2010-12-03       Impact factor: 0.972

4.  Proteins and lipids define the diffusional field of nitric oxide.

Authors:  D M Porterfield; J D Laskin; S K Jung; R P Malchow; B Billack; P J Smith; D E Heck
Journal:  Am J Physiol Lung Cell Mol Physiol       Date:  2001-10       Impact factor: 5.464

5.  Nitric oxide-mediated bystander effect induced by heavy-ions in human salivary gland tumour cells.

Authors:  C Shao; Y Furusawa; M Aoki; H Matsumoto; K Ando
Journal:  Int J Radiat Biol       Date:  2002-09       Impact factor: 2.694

6.  Novel neutron sources at the Radiological Research Accelerator Facility.

Authors:  Yanping Xu; Guy Garty; Stephen A Marino; Thomas N Massey; Gerhard Randers-Pehrson; Gary W Johnson; David J Brenner
Journal:  J Instrum       Date:  2012-03-16       Impact factor: 1.415

7.  The Columbia University Sub-micron Charged Particle Beam.

Authors:  Gerhard Randers-Pehrson; Gary W Johnson; Stephen A Marino; Yanping Xu; Alexander D Dymnikov; David J Brenner
Journal:  Nucl Instrum Methods Phys Res A       Date:  2009-10-11       Impact factor: 1.455

8.  Oxidative metabolism modulates signal transduction and micronucleus formation in bystander cells from alpha-particle-irradiated normal human fibroblast cultures.

Authors:  Edouard I Azzam; Sonia M De Toledo; Douglas R Spitz; John B Little
Journal:  Cancer Res       Date:  2002-10-01       Impact factor: 12.701

Review 9.  Advances in radiobiological studies using a microbeam.

Authors:  Tom K Hei; Leslie K Ballas; David J Brenner; Charles R Geard
Journal:  J Radiat Res       Date:  2009-03       Impact factor: 2.724

10.  The self-referencing oxygen-selective microelectrode: detection of transmembrane oxygen flux from single cells.

Authors:  S C Land; D M Porterfield; R H Sanger; P J Smith
Journal:  J Exp Biol       Date:  1999-01       Impact factor: 3.312
  10 in total
  2 in total

1.  Real-time local oxygen measurements for high resolution cellular imaging.

Authors:  Liron Boyman; George S B Williams; Andrew P Wescott; Jennie B Leach; Joseph P Y Kao; W Jonathan Lederer
Journal:  J Mol Cell Cardiol       Date:  2018-12-05       Impact factor: 5.000

2.  A Horizontal Multi-Purpose Microbeam System.

Authors:  Y Xu; G Randers-Pehrson; S A Marino; G Garty; A Harken; D J Brenner
Journal:  Nucl Instrum Methods Phys Res A       Date:  2018-02-04       Impact factor: 1.455
  2 in total

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