Literature DB >> 2532965

Bialaphos resistance as a dominant selectable marker in Neurospora crassa.

J Avalos1, R F Geever, M E Case.   

Abstract

Conidia of Neurospora crassa are sensitive to the herbicide bialaphos at concentrations of 160 mg/l in Westergaard's or Fries' minimal media. Plasmid pJA4 was constructed by inserting a truncated bar gene from Streptomyces hygroscopicus fused to the his-3 promoter from N. crassa into pUC19. The bar gene in plasmid pJA4 confers resistance to bialaphos when transformants are selected on a medium containing bialaphos. The bar gene can be used as an additional dominant selectable marker for transformation of fungi.

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Year:  1989        PMID： 2532965     DOI： 10.1007/bf00340716

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  9 in total

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Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Cloning and characterization of the multifunctional his-3 gene of Neurospora crassa.

Authors:  T L Legerton; C Yanofsky
Journal:  Gene       Date:  1985       Impact factor: 3.688

3.  Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

Authors:  M Dagert; S D Ehrlich
Journal:  Gene       Date:  1979-05       Impact factor: 3.688

4.  Efficient cloning of genes of Neurospora crassa.

Authors:  S J Vollmer; C Yanofsky
Journal:  Proc Natl Acad Sci U S A       Date:  1986-07       Impact factor: 11.205

5.  Transformation of Aspergillus nidulans by using a trpC plasmid.

Authors:  M M Yelton; J E Hamer; W E Timberlake
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

6.  Efficient transformation of Neurospora crassa by utilizing hybrid plasmid DNA.

Authors:  M E Case; M Schweizer; S R Kushner; N H Giles
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

7.  Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker.

Authors:  M J Orbach; E B Porro; C Yanofsky
Journal:  Mol Cell Biol       Date:  1986-07       Impact factor: 4.272

8.  Engineering herbicide resistance in plants by expression of a detoxifying enzyme.

Authors:  M D Block; J Botterman; M Vandewiele; J Dockx; C Thoen; V Gosselé; N R Movva; C Thompson; M V Montagu; J Leemans
Journal:  EMBO J       Date:  1987-09       Impact factor: 11.598

9.  Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus.

Authors:  C J Thompson; N R Movva; R Tizard; R Crameri; J E Davies; M Lauwereys; J Botterman
Journal:  EMBO J       Date:  1987-09       Impact factor: 11.598
  9 in total
  16 in total

1.  Transformation of the Fungal Soybean Pathogen Cercospora kikuchii with the Selectable Marker bar.

Authors:  R G Upchurch; M J Meade; R C Hightower; R S Thomas; T M Callahan
Journal:  Appl Environ Microbiol       Date:  1994-12       Impact factor: 4.792

Review 2.  Review: Global nutrient profiling by Phenotype MicroArrays: a tool complementing genomic and proteomic studies in conidial fungi.

Authors:  Lea Atanasova; Irina S Druzhinina
Journal:  J Zhejiang Univ Sci B       Date:  2010-03       Impact factor: 3.066

3.  Normal Patterns of Histone H3K27 Methylation Require the Histone Variant H2A.Z in Neurospora crassa.

Authors:  Abigail J Courtney; Masayuki Kamei; Aileen R Ferraro; Kexin Gai; Qun He; Shinji Honda; Zachary A Lewis
Journal:  Genetics       Date:  2020-07-10       Impact factor: 4.562

4.  Induction of H3K9me3 and DNA methylation by tethered heterochromatin factors in Neurospora crassa.

Authors:  Jordan D Gessaman; Eric U Selker
Journal:  Proc Natl Acad Sci U S A       Date:  2017-10-23       Impact factor: 11.205

5.  Global analysis of serine-threonine protein kinase genes in Neurospora crassa.

Authors:  Gyungsoon Park; Jacqueline A Servin; Gloria E Turner; Lorena Altamirano; Hildur V Colot; Patrick Collopy; Liubov Litvinkova; Liande Li; Carol A Jones; Fitz-Gerald Diala; Jay C Dunlap; Katherine A Borkovich
Journal:  Eukaryot Cell       Date:  2011-09-30

6.  Homologous expression of Phanerochaete chrysosporium manganese peroxidase, using bialaphos resistance as a dominant selectable marker.

Authors:  Biao Ma; Mary B Mayfield; Michael H Gold
Journal:  Curr Genet       Date:  2003-07-03       Impact factor: 3.886

7.  Cercosporin-deficient mutants by plasmid tagging in the asexual fungus Cercospora nicotianae.

Authors:  K-R Chung; M Ehrenshaft; D K Wetzel; M E Daub
Journal:  Mol Genet Genomics       Date:  2003-08-21       Impact factor: 3.291

8.  High efficiency transformation of Tolypocladium geodes conidiospores to phleomycin resistance.

Authors:  T Calmels; M Parriche; H Durand; G Tiraby
Journal:  Curr Genet       Date:  1991-09       Impact factor: 3.886

9.  Complexity generation in fungal peptidyl alkaloid biosynthesis: a two-enzyme pathway to the hexacyclic MDR export pump inhibitor ardeemin.

Authors:  Stuart W Haynes; Xue Gao; Yi Tang; Christopher T Walsh
Journal:  ACS Chem Biol       Date:  2013-02-06       Impact factor: 5.100

10.  Global nutritional profiling for mutant and chemical mode-of-action analysis in filamentous fungi.

Authors:  Matthew M Tanzer; Herbert N Arst; Amy R Skalchunes; Marie Coffin; Blaise A Darveaux; Ryan W Heiniger; Jeffrey R Shuster
Journal:  Funct Integr Genomics       Date:  2003-07-30       Impact factor: 3.410
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