Literature DB >> 16349469

Transformation of the Fungal Soybean Pathogen Cercospora kikuchii with the Selectable Marker bar.

R G Upchurch1, M J Meade, R C Hightower, R S Thomas, T M Callahan.   

Abstract

An improved transformation protocol, utilizing selection for resistance to the herbicide bialaphos, has been developed for the plant pathogenic fungus Cercospora kikuchii. Stable, bialaphos-resistant transformants are recovered at frequencies eight times higher than those achieved with the previous system that was based on selection for benomyl resistance. In addition to C. kikuchii, this improved method can also be used to transform other species of Cercospora.

Entities:  

Year:  1994        PMID： 16349469      PMCID： PMC202025          DOI： 10.1128/aem.60.12.4592-4595.1994

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  7 in total

1.  Mutants of Cercospora kikuchii Altered in Cercosporin Synthesis and Pathogenicity.

Authors:  R G Upchurch; D C Walker; J A Rollins; M Ehrenshaft; M E Daub
Journal:  Appl Environ Microbiol       Date:  1991-10       Impact factor: 4.792

2.  Isolation of Light-Enhanced cDNAs of Cercospora kikuchii.

Authors:  M Ehrenshaft; R G Upchurch
Journal:  Appl Environ Microbiol       Date:  1991-09       Impact factor: 4.792

3.  Genetic Transformation System for the Fungal Soybean Pathogen Cercospora kikuchii.

Authors:  R G Upchurch; M Ehrenshaft; D C Walker; L A Sanders
Journal:  Appl Environ Microbiol       Date:  1991-10       Impact factor: 4.792

4.  A small cosmid for efficient cloning of large DNA fragments.

Authors:  B Hohn; J Collins
Journal:  Gene       Date:  1980-11       Impact factor: 3.688

5.  Expression of the Escherichia coli beta-glucuronidase gene in industrial and phytopathogenic filamentous fungi.

Authors:  I N Roberts; R P Oliver; P J Punt; C A van den Hondel
Journal:  Curr Genet       Date:  1989-03       Impact factor: 3.886

6.  Bialaphos resistance as a dominant selectable marker in Neurospora crassa.

Authors:  J Avalos; R F Geever; M E Case
Journal:  Curr Genet       Date:  1989-12       Impact factor: 3.886

7.  Engineering herbicide resistance in plants by expression of a detoxifying enzyme.

Authors:  M D Block; J Botterman; M Vandewiele; J Dockx; C Thoen; V Gosselé; N R Movva; C Thompson; M V Montagu; J Leemans
Journal:  EMBO J       Date:  1987-09       Impact factor: 11.598
  7 in total
  1 in total

1.  Homologous expression of Phanerochaete chrysosporium manganese peroxidase, using bialaphos resistance as a dominant selectable marker.

Authors:  Biao Ma; Mary B Mayfield; Michael H Gold
Journal:  Curr Genet       Date:  2003-07-03       Impact factor: 3.886
  1 in total

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