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Literature DB >> 16453789

Engineering herbicide resistance in plants by expression of a detoxifying enzyme.

M D Block¹, J Botterman, M Vandewiele, J Dockx, C Thoen, V Gosselé, N R Movva, C Thompson, M V Montagu, J Leemans.

Abstract

Phosphinothricin (PPT) is a potent inhibitor of glutamine synthetase in plants and is used as a non-selective herbicide. The bar gene which confers resistance in Streptomyces hygroscopicus to bialaphos, a tripeptide containing PPT, encodes a phosphinothricin acetyltransferase (PAT) (see accompanying paper). The bar gene was placed under control of the 35S promoter of the cauliflower mosaic virus and transferred to plant cells using Agrobacterium-mediated transformation. PAT was used as a selectable marker in protoplast co-cultivation. The chimeric bar gene was expressed in tobacco, potato and tomato plants. Transgenic plants showed complete resistance towards high doses of the commercial formulations of phosphinothricin and bialaphos. These data present a successful approach to obtain herbicide-resistant plants by detoxification of the herbicide.

Entities: Chemical

Year: 1987 PMID： 16453789 PMCID： PMC553667 DOI： 10.1002/j.1460-2075.1987.tb02537.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

18 in total

1. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria.

Authors: W V Shaw
Journal: Methods Enzymol Date: 1975 Impact factor: 1.600

2. Streptomycin-resistant plants from callus culture of haploid tobacco.

Authors: P Maliga; A Sz-Breznovits; L Márton
Journal: Nat New Biol Date: 1973-07-04

3. [Metabolic products of microorganisms. 98. Phosphinothricin and phosphinothricyl-alanyl-analine].

Authors: E Bayer; K H Gugel; K Hägele; H Hagenmaier; S Jessipow; W A König; H Zähner
Journal: Helv Chim Acta Date: 1972-01-31 Impact factor: 2.164

4. Nutrient requirements of suspension cultures of soybean root cells.

Authors: O L Gamborg; R A Miller; K Ojima
Journal: Exp Cell Res Date: 1968-04 Impact factor: 3.905

5. Herbicide-resistant alfalfa cells: an example of gene amplification in plants.

Authors: G Donn; E Tischer; J A Smith; H M Goodman
Journal: J Mol Appl Genet Date: 1984

6. Plant glutamine synthetase complements a glnA mutation in Escherichia coli.

Authors: S DasSarma; E Tischer; H M Goodman
Journal: Science Date: 1986-06-06 Impact factor: 47.728

7. Selection-expression plasmid vectors for use in genetic transformation of higher plants.

Authors: J Velten; J Schell
Journal: Nucleic Acids Res Date: 1985-10-11 Impact factor: 16.971

8. Comparison of cauliflower mosaic virus 35S and nopaline synthase promoters in transgenic plants.

Authors: P R Sanders; J A Winter; A R Barnason; S G Rogers; R T Fraley
Journal: Nucleic Acids Res Date: 1987-02-25 Impact factor: 16.971

9. Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants.

Authors: R Deblaere; B Bytebier; H De Greve; F Deboeck; J Schell; M Van Montagu; J Leemans
Journal: Nucleic Acids Res Date: 1985-07-11 Impact factor: 16.971

10. Glutathione conjugation. An enzymatic basis for atrazine resistance in corn.

Authors: R H Shimabukuro; D S Frear; H R Swanson; W C Walsh
Journal: Plant Physiol Date: 1971-01 Impact factor: 8.340

177 in total

1. A four-element based transposon system for allele specific tagging in plants--theoretical considerations.

Authors: S Phogat; P K Burma; D Pental
Journal: J Biosci Date: 2000-03 Impact factor: 1.826

2. Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns.

Authors: X Fu; L T Duc; S Fontana; B B Bong; P Tinjuangjun; D Sudhakar; R M Twyman; P Christou; A Kohli
Journal: Transgenic Res Date: 2000-02 Impact factor: 2.788

3. The late developmental pattern of Mu transposon excision is conferred by a cauliflower mosaic virus 35S -driven MURA cDNA in transgenic maize.

Authors: M N Raizada; V Walbot
Journal: Plant Cell Date: 2000-01 Impact factor: 11.277

Engineering herbicide resistance in plants by expression of a detoxifying enzyme.

1. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria.

2. Streptomycin-resistant plants from callus culture of haploid tobacco.

3. [Metabolic products of microorganisms. 98. Phosphinothricin and phosphinothricyl-alanyl-analine].

4. Nutrient requirements of suspension cultures of soybean root cells.

5. Herbicide-resistant alfalfa cells: an example of gene amplification in plants.

6. Plant glutamine synthetase complements a glnA mutation in Escherichia coli.

7. Selection-expression plasmid vectors for use in genetic transformation of higher plants.

8. Comparison of cauliflower mosaic virus 35S and nopaline synthase promoters in transgenic plants.

9. Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants.

10. Glutathione conjugation. An enzymatic basis for atrazine resistance in corn.

1. A four-element based transposon system for allele specific tagging in plants--theoretical considerations.

2. Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns.

3. The late developmental pattern of Mu transposon excision is conferred by a cauliflower mosaic virus 35S -driven MURA cDNA in transgenic maize.

4. pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation.

Review 5. Gene traps: tools for plant development and genomics.

6. Use of epitope tags for routine analysis of transgene expression.

7. Cre recombinase expression can result in phenotypic aberrations in plants.

8. An active role for endogenous beta-1,3-glucanase genes in transgene-mediated co-suppression in tobacco.

9. Plant Expression of a Bacterial Cytochrome P450 That Catalyzes Activation of a Sulfonylurea Pro-Herbicide.

10. Generation of Large Numbers of Independently Transformed Fertile Barley Plants.