| Literature DB >> 25303531 |
Jill M Dowen1, Zi Peng Fan1,2, Denes Hnisz1, Gang Ren3,4, Brian J Abraham1, Lyndon N Zhang1,5, Abraham S Weintraub1,5, Jurian Schujiers1, Tong Ihn Lee1, Keji Zhao3, Richard A Young1,5.
Abstract
The pluripotent state of embryonic stem cells (ESCs) is produced by active transcription of genes that control cell identity and repression of genes encoding lineage-specifying developmental regulators. Here, we use ESC cohesin ChIA-PET data to identify the local chromosomal structures at both active and repressed genes across the genome. The results produce a map of enhancer-promoter interactions and reveal that super-enhancer-driven genes generally occur within chromosome structures that are formed by the looping of two interacting CTCF sites co-occupied by cohesin. These looped structures form insulated neighborhoods whose integrity is important for proper expression of local genes. We also find that repressed genes encoding lineage-specifying developmental regulators occur within insulated neighborhoods. These results provide insights into the relationship between transcriptional control of cell identity genes and control of local chromosome structure.Entities:
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Year: 2014 PMID： 25303531 PMCID： PMC4197132 DOI： 10.1016/j.cell.2014.09.030
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582