Literature DB >> 2526319

Labelling oligonucleotides to high specific activity (I).

A G Craig1, D Nizetic, H Lehrach.   

Abstract

The normal procedure for labelling oligonucleotides radioactively is the use of polynucleotide kinase and gamma 32P-ATP. However, this has the disadvantage of only introducing one labelled base per molecule of the oligonucleotide. In this paper we describe an approach based on primer/template combinations using conventional fill-in conditions followed by the release of the labelled sequence by digestion with uracil-DNA glycosylase.

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Year:  1989        PMID： 2526319      PMCID： PMC318018          DOI： 10.1093/nar/17.12.4605

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

1.  Discrimination among the transcripts of the allelic human beta-globin genes beta A, beta S and beta C using oligodeoxynucleotide hybridization probes.

Authors:  G Nozari; S Rahbar; R B Wallace
Journal:  Gene       Date:  1986       Impact factor: 3.688

2.  Thermodynamic studies of base pairing involving 2,6-diaminopurine.

Authors:  C Cheong; I Tinoco; A Chollet
Journal:  Nucleic Acids Res       Date:  1988-06-10       Impact factor: 16.971

3.  DNA N-glycosidases: properties of uracil-DNA glycosidase from Escherichia coli.

Authors:  T Lindahl; S Ljungquist; W Siegert; B Nyberg; B Sperens
Journal:  J Biol Chem       Date:  1977-05-25       Impact factor: 5.157

4.  DNA glycosylases, endonucleases for apurinic/apyrimidinic sites, and base excision-repair.

Authors:  T Lindahl
Journal:  Prog Nucleic Acid Res Mol Biol       Date:  1979

5.  Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.

Authors:  W I Wood; J Gitschier; L A Lasky; R M Lawn
Journal:  Proc Natl Acad Sci U S A       Date:  1985-03       Impact factor: 11.205

6.  Genomic sequencing.

Authors:  G M Church; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

7.  Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates.

Authors:  J F Milligan; D R Groebe; G W Witherell; O C Uhlenbeck
Journal:  Nucleic Acids Res       Date:  1987-11-11       Impact factor: 16.971

8.  Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch.

Authors:  R B Wallace; J Shaffer; R F Murphy; J Bonner; T Hirose; K Itakura
Journal:  Nucleic Acids Res       Date:  1979-08-10       Impact factor: 16.971

9.  DNA finger printing by oligonucleotide probes specific for simple repeats.

Authors:  S Ali; C R Müller; J T Epplen
Journal:  Hum Genet       Date:  1986-11       Impact factor: 4.132
  9 in total
  2 in total

1.  Contrasting effects of single stranded DNA binding protein on the activity of uracil DNA glycosylase from Escherichia coli towards different DNA substrates.

Authors:  N V Kumar; U Varshney
Journal:  Nucleic Acids Res       Date:  1997-06-15       Impact factor: 16.971

2.  Inefficient excision of uracil from loop regions of DNA oligomers by E. coli uracil DNA glycosylase.

Authors:  N V Kumar; U Varshney
Journal:  Nucleic Acids Res       Date:  1994-09-11       Impact factor: 16.971
  2 in total

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