PURPOSE: To validate a novel and more practical system for trophectoderm DNA fingerprinting which reliably distinguishes sibling embryos from each other. METHODS: In this prospective and blinded study two-cell and 5-cell samples from commercially available sibling cell lines and excess DNA from trophectoderm biopsies of sibling human blastocysts were evaluated for accurate assignment of relationship using qPCR-based allelic discrimination from 40 single nucleotide polymorphisms (SNPs) with low allele frequency variation and high heterozygosity. RESULTS: Cell samples with self relationships averaged 95.1 ± 5.9 % similarity. Sibling relationships averaged 57.2 ± 5.9 % similarity for all 40 SNPs, and 40.8 ± 8.2 % similarity for the 25 informative SNPs. Assignment of relationships was accomplished with 100 % accuracy for cell lines and embryos. CONCLUSIONS: These data demonstrate the first trophectoderm qPCR-based DNA fingerprinting technology capable of unequivocal discrimination of sibling human embryos. This methodology will empower research and development of new markers of, and interventions that influence embryonic reproductive potential.
PURPOSE: To validate a novel and more practical system for trophectoderm DNA fingerprinting which reliably distinguishes sibling embryos from each other. METHODS: In this prospective and blinded study two-cell and 5-cell samples from commercially available sibling cell lines and excess DNA from trophectoderm biopsies of sibling humanblastocysts were evaluated for accurate assignment of relationship using qPCR-based allelic discrimination from 40 single nucleotide polymorphisms (SNPs) with low allele frequency variation and high heterozygosity. RESULTS: Cell samples with self relationships averaged 95.1 ± 5.9 % similarity. Sibling relationships averaged 57.2 ± 5.9 % similarity for all 40 SNPs, and 40.8 ± 8.2 % similarity for the 25 informative SNPs. Assignment of relationships was accomplished with 100 % accuracy for cell lines and embryos. CONCLUSIONS: These data demonstrate the first trophectoderm qPCR-based DNA fingerprinting technology capable of unequivocal discrimination of sibling human embryos. This methodology will empower research and development of new markers of, and interventions that influence embryonic reproductive potential.
Authors: Nathan R Treff; Jing Su; Natasha Kasabwala; Xin Tao; Kathleen A Miller; Richard T Scott Journal: Fertil Steril Date: 2009-11-06 Impact factor: 7.329
Authors: Nathan R Treff; Xin Tao; Kathleen M Ferry; Jing Su; Deanne Taylor; Richard T Scott Journal: Fertil Steril Date: 2012-02-18 Impact factor: 7.329
Authors: Nathan R Treff; Xin Tao; Jing Su; Agnieszka Lonczak; Lesley E Northrop; Andrew A Ruiz; Richard T Scott Journal: Mol Hum Reprod Date: 2011-02-28 Impact factor: 4.025
Authors: Daniela Bettio; Antonio Capalbo; Elena Albani; Laura Rienzi; Valentina Achille; Anna Venci; Filippo Maria Ubaldi; Paolo Emanuele Levi Setti Journal: Reprod Biol Endocrinol Date: 2016-09-06 Impact factor: 5.211