OBJECTIVE: To validate a novel system for embryonic DNA fingerprinting which can reliably distinguish sibling embryos from each other. DESIGN: Prospective, randomized, and blinded study. SETTING:Academic center for reproductive medicine. PATIENT(S): Blastomeres were obtained from discarded and transferred embryos from six patients undergoing IVF treatment. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Single lymphocytes from sibling cell lines and blastomeres from sibling day 3 human embryos were evaluated for accurate assignment of relationship using whole genome amplification and single-nucleotide polymorphism microarrays. RESULT(S): Assignment of single-cell relationships was accomplished with 100% accuracy. We also observed complete agreement between the molecular karyotype and DNA fingerprint-based identification of embryos implanted in three clinical IVF cases after multiple embryo transfer. CONCLUSION(S): These data demonstrate the first single-blastomere DNA fingerprinting technology capable of unequivocal discrimination of sibling human embryos. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
RCT Entities:
OBJECTIVE: To validate a novel system for embryonic DNA fingerprinting which can reliably distinguish sibling embryos from each other. DESIGN: Prospective, randomized, and blinded study. SETTING: Academic center for reproductive medicine. PATIENT(S): Blastomeres were obtained from discarded and transferred embryos from six patients undergoing IVF treatment. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Single lymphocytes from sibling cell lines and blastomeres from sibling day 3 human embryos were evaluated for accurate assignment of relationship using whole genome amplification and single-nucleotide polymorphism microarrays. RESULT(S): Assignment of single-cell relationships was accomplished with 100% accuracy. We also observed complete agreement between the molecular karyotype and DNA fingerprint-based identification of embryos implanted in three clinical IVF cases after multiple embryo transfer. CONCLUSION(S): These data demonstrate the first single-blastomere DNA fingerprinting technology capable of unequivocal discrimination of sibling human embryos. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Authors: Richard T Scott; Nathan R Treff; John Stevens; Eric J Forman; Kathleen H Hong; Mandy G Katz-Jaffe; William B Schoolcraft Journal: J Assist Reprod Genet Date: 2012-03-30 Impact factor: 3.412