Literature DB >> 2502516

Construction of mouse A9 clones containing a single human chromosome tagged with neomycin-resistance gene via microcell fusion.

M Koi1, M Shimizu, H Morita, H Yamada, M Oshimura.   

Abstract

Normal human fibroblasts (MRC-5 or NTI-4) were transfected with pSV2-neo plasmid DNA. Fifty G418-resistant fibroblast clones were isolated and independently fused to mouse A9 cells. The cell hybrids were selected and isolated in the medium containing G418 plus ouabain. Since micronuclei were more efficiently induced in these hybrids compared to parental human fibroblasts by colcemid treatment, the transfer of neo-tagged human chromosomes in the hybrids to mouse A9 cells was performed via microcell fusion. Two hundred A9 microcell hybrids were isolated and karyotyped. Among them, thirteen microcell clones, each containing a single human chromosome 1, 2, 5, 6, 7, 8, 10, 11, 12, 15, 18, 19 or 20 were established. Isozyme analyses conformed the presence of each human chromosome in these A9 microcell clones. The results of Southern blot and chromosomal in situ hybridization analyses indicate that the human chromosomes in these clones were tagged with pSV2-neo plasmid DNA.

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Year:  1989        PMID： 2502516      PMCID： PMC5917765          DOI： 10.1111/j.1349-7006.1989.tb02329.x

Source DB:  PubMed          Journal:  Jpn J Cancer Res        ISSN： 0910-5050


  12 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Introduction of a normal human chromosome 11 into a Wilms' tumor cell line controls its tumorigenic expression.

Authors:  B E Weissman; P J Saxon; S R Pasquale; G R Jones; A G Geiser; E J Stanbridge
Journal:  Science       Date:  1987-04-10       Impact factor: 47.728

3.  Loss of tumor-suppressive function during chemically induced neoplastic progression of Syrian hamster embryo cells.

Authors:  M Koi; J C Barrett
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205

4.  Selective transfer of individual human chromosomes to recipient cells.

Authors:  P J Saxon; E S Srivatsan; G V Leipzig; J H Sameshima; E J Stanbridge
Journal:  Mol Cell Biol       Date:  1985-01       Impact factor: 4.272

5.  Nuclear control of tumorigenicity in cells reconstructed by PEG-induced fusion of cell fragments.

Authors:  J W Shay; M A Clark
Journal:  J Supramol Struct       Date:  1979

6.  Localization of the human insulin gene to the distal end of the short arm of chromosome 11.

Authors:  M E Harper; A Ullrich; G F Saunders
Journal:  Proc Natl Acad Sci U S A       Date:  1981-07       Impact factor: 11.205

7.  Normal human chromosome 11 suppresses tumorigenicity of human cervical tumor cell line SiHa.

Authors:  M Koi; H Morita; H Yamada; H Satoh; J C Barrett; M Oshimura
Journal:  Mol Carcinog       Date:  1989       Impact factor: 4.784

8.  Integration of a dominant selectable marker into human chromosomes and transfer of marked chromosomes to mouse cells by microcell fusion.

Authors:  R S Athwal; M Smarsh; B M Searle; S S Deo
Journal:  Somat Cell Mol Genet       Date:  1985-03

9.  Introduction of human chromosome 11 via microcell transfer controls tumorigenic expression of HeLa cells.

Authors:  P J Saxon; E S Srivatsan; E J Stanbridge
Journal:  EMBO J       Date:  1986-12-20       Impact factor: 11.598

10.  Construction of mouse A9 clones containing a single human chromosome (X/autosome translocation) via micro-cell fusion.

Authors:  M Koi; H Morita; M Shimizu; M Oshimura
Journal:  Jpn J Cancer Res       Date:  1989-02
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  39 in total

1.  Brca2 (XRCC11) deficiency results in radioresistant DNA synthesis and a higher frequency of spontaneous deletions.

Authors:  Maria Kraakman-van der Zwet; Wilhelmina J I Overkamp; Rebecca E E van Lange; Jeroen Essers; Annemarie van Duijn-Goedhart; Ingrid Wiggers; Srividya Swaminathan; Paul P W van Buul; Abdellatif Errami; Raoul T L Tan; Nicolaas G J Jaspers; Shyam K Sharan; Roland Kanaar; Malgorzata Z Zdzienicka
Journal:  Mol Cell Biol       Date:  2002-01       Impact factor: 4.272

Review 2.  Human artificial chromosomes for gene delivery and the development of animal models.

Authors:  Yasuhiro Kazuki; Mitsuo Oshimura
Journal:  Mol Ther       Date:  2011-07-12       Impact factor: 11.454

3.  Chromosome-band-specific painting: chromosome in situ suppression hybridization using PCR products from a microdissected chromosome band as a probe pool.

Authors:  H X Deng; K Yoshiura; R W Dirks; N Harada; T Hirota; K Tsukamoto; Y Jinno; N Niikawa
Journal:  Hum Genet       Date:  1992-04       Impact factor: 4.132

4.  Position of human chromosomes is conserved in mouse nuclei indicating a species-independent mechanism for maintaining genome organization.

Authors:  Kundan Sengupta; Jordi Camps; Priya Mathews; Linda Barenboim-Stapleton; Quang Tri Nguyen; Michael J Difilippantonio; Thomas Ried
Journal:  Chromosoma       Date:  2008-06-18       Impact factor: 4.316

5.  XR-C1, a new CHO cell mutant which is defective in DNA-PKcs, is impaired in both V(D)J coding and signal joint formation.

Authors:  A Errami; D M He; A A Friedl; W J Overkamp; B Morolli; E A Hendrickson; F Eckardt-Schupp; M Oshimura; P H Lohman; S P Jackson; M Z Zdzienicka
Journal:  Nucleic Acids Res       Date:  1998-07-01       Impact factor: 16.971

6.  Human complement regulatory proteins expressed on mouse A9 cells containing a human chromosome 1.

Authors:  T Seya; M Okada; T Hara; M Matsumoto; S Miyagawa; M Oshimura
Journal:  Immunology       Date:  1991-12       Impact factor: 7.397

7.  Neuron-specific impairment of inter-chromosomal pairing and transcription in a novel model of human 15q-duplication syndrome.

Authors:  Makiko Meguro-Horike; Dag H Yasui; Weston Powell; Diane I Schroeder; Mitsuo Oshimura; Janine M Lasalle; Shin-ichi Horike
Journal:  Hum Mol Genet       Date:  2011-07-01       Impact factor: 6.150

8.  The transfer of human artificial chromosomes via cryopreserved microcells.

Authors:  Narumi Uno; Katsuhiro Uno; Susi Zatti; Kana Ueda; Masaharu Hiratsuka; Motonobu Katoh; Mitsuo Oshimura
Journal:  Cytotechnology       Date:  2013-03-15       Impact factor: 2.058

Review 9.  A new generation of human artificial chromosomes for functional genomics and gene therapy.

Authors:  Natalay Kouprina; William C Earnshaw; Hiroshi Masumoto; Vladimir Larionov
Journal:  Cell Mol Life Sci       Date:  2012-08-21       Impact factor: 9.261

10.  Human chromosome 11 complements ataxia-telangiectasia cells but does not complement the defect in AT-like Chinese hamster cell mutants.

Authors:  W Jongmans; J Wiegant; M Oshimura; M R James; P H Lohman; M Z Zdzienicka
Journal:  Hum Genet       Date:  1993-10-01       Impact factor: 4.132
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