| Literature DB >> 25002145 |
Weiqiang Qian1, Daisuke Miki2, Mingguang Lei3, Xiaohong Zhu3, Huiming Zhang3, Yunhua Liu3, Yan Li2, Zhaobo Lang3, Jing Wang2, Kai Tang3, Renyi Liu2, Jian-Kang Zhu4.
Abstract
DNA methylation patterns are dynamically controlled by DNA methylation and active DNA demethylation, but the mechanisms of regulation of active DNA demethylation are not well understood. Through forward genetic screens for Arabidopsis mutants showing DNA hypermethylation at specific loci and increased silencing of reporter genes, we identified IDM2 (increased DNA methylation 2) as a regulator of DNA demethylation and gene silencing. IDM2 dysfunction causes DNA hypermethylation and silencing of reporter genes and some endogenous genes. These effects of idm2 mutations are similar to those of mutations in IDM1, a regulator of active DNA demethylation. IDM2 encodes an α-crystallin domain protein in the nucleus. IDM2 and IDM1 interact physically and partially colocalize at discrete subnuclear foci. IDM2 is required for the full activity of H3K18 acetylation but not H3K23 acetylation of IDM1 in planta. Our results suggest that IDM2 functions in active DNA demethylation and in antisilencing by regulating IDM1.Entities:
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Year: 2014 PMID： 25002145 PMCID： PMC4302764 DOI： 10.1016/j.molcel.2014.06.008
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970