Literature DB >> 24947398

Probing the role of the actin cytoskeleton during regulated exocytosis by intravital microscopy.

Oleg Milberg1, Muhibullah Tora, Akiko Shitara, Taishin Takuma, Andrius Masedunskas, Roberto Weigert.   

Abstract

The actin cytoskeleton plays a fundamental role in controlling several steps during regulated exocytosis. Here, we describe a combination of procedures that are aimed at studying the dynamics and the mechanism of the actin cytoskeleton in the salivary glands of live rodents, a model for exocrine secretion. Our approach relies on intravital microscopy, an imaging technique that enables imaging biological events in live animals at a subcellular resolution, and it is complemented by the use of pharmacological agents and indirect immunofluorescence in the salivary tissue.

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Year:  2014        PMID： 24947398      PMCID： PMC4699287          DOI： 10.1007/978-1-4939-0944-5_28

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


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