| Literature DB >> 18536722 |
Julia Riedl1, Alvaro H Crevenna, Kai Kessenbrock, Jerry Haochen Yu, Dorothee Neukirchen, Michal Bista, Frank Bradke, Dieter Jenne, Tad A Holak, Zena Werb, Michael Sixt, Roland Wedlich-Soldner.
Abstract
Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.Mesh：
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Year: 2008 PMID： 18536722 PMCID： PMC2814344 DOI： 10.1038/nmeth.1220
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547