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Literature DB >> 24890431

Improving site-directed RNA editing in vitro and in cell culture by chemical modification of the guideRNA.

Paul Vogel¹, Marius F Schneider, Jacqueline Wettengel, Thorsten Stafforst.

Abstract

Adenosine-to-inosine deamination can be re-addressed to user-defined mRNAs by applying phosphothioate/2'-methoxy-modified guideRNAs. Dense chemical modification of the guideRNA clearly improves performance of the covalent conjugates inside the living cell. Furthermore, careful positioning of a few modifications controls editing selectivity in vitro and was exploited for the challenging repair of the Factor 5 Leiden missense mutation.

Entities: Chemical

Keywords: RNA editing; antagomirs; genetic diseases; nucleic acid analogues; transcript repair

Mesh：

Substances：
RNA

Year: 2014 PMID： 24890431 DOI： 10.1002/anie.201402634

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

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Cited

29 in total

Review 1. Current strategies for Site-Directed RNA Editing using ADARs.

Authors: Maria Fernanda Montiel-Gonzalez; Juan Felipe Diaz Quiroz; Joshua J C Rosenthal
Journal: Methods Date: 2018-11-29 Impact factor: 3.608

Review 2. Optochemical Control of Biological Processes in Cells and Animals.

Authors: Nicholas Ankenbruck; Taylor Courtney; Yuta Naro; Alexander Deiters
Journal: Angew Chem Int Ed Engl Date: 2018-02-01 Impact factor: 15.336

3. A cytosine deaminase for programmable single-base RNA editing.

Authors: Omar O Abudayyeh; Jonathan S Gootenberg; Brian Franklin; Jeremy Koob; Max J Kellner; Alim Ladha; Julia Joung; Paul Kirchgatterer; David B T Cox; Feng Zhang
Journal: Science Date: 2019-07-11 Impact factor: 47.728

4. REPAIRx, a specific yet highly efficient programmable A > I RNA base editor.

Authors: Yajing Liu; Shaoshuai Mao; Shisheng Huang; Yongqin Li; Yuxin Chen; Minghui Di; Xinxin Huang; Junjun Lv; Xinxin Wang; Jianyang Ge; Shengxi Shen; Xiaoming Zhang; Dahai Liu; Xingxu Huang; Tian Chi
Journal: EMBO J Date: 2020-10-15 Impact factor: 11.598

5. Abundant off-target edits from site-directed RNA editing can be reduced by nuclear localization of the editing enzyme.

Authors: Isabel C Vallecillo-Viejo; Noa Liscovitch-Brauer; Maria Fernanda Montiel-Gonzalez; Eli Eisenberg; Joshua J C Rosenthal
Journal: RNA Biol Date: 2017-11-13 Impact factor: 4.652

Improving site-directed RNA editing in vitro and in cell culture by chemical modification of the guideRNA.

Review 1. Current strategies for Site-Directed RNA Editing using ADARs.

Review 2. Optochemical Control of Biological Processes in Cells and Animals.

3. A cytosine deaminase for programmable single-base RNA editing.

4. REPAIRx, a specific yet highly efficient programmable A > I RNA base editor.

5. Abundant off-target edits from site-directed RNA editing can be reduced by nuclear localization of the editing enzyme.

Review 6. Editing the Genome Without Double-Stranded DNA Breaks.

7. Site-directed RNA repair of endogenous Mecp2 RNA in neurons.

Review 8. Base editing: precision chemistry on the genome and transcriptome of living cells.

9. Rational Design of RNA Editing Guide Strands: Cytidine Analogs at the Orphan Position.

10. TRIBE: Hijacking an RNA-Editing Enzyme to Identify Cell-Specific Targets of RNA-Binding Proteins.