Literature DB >> 24880488

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system.

Mehdi Ghorbal1, Molly Gorman2, Cameron Ross Macpherson2, Rafael Miyazawa Martins2, Artur Scherf2, Jose-Juan Lopez-Rubio1.   

Abstract

Genome manipulation in the malaria parasite Plasmodium falciparum remains largely intractable and improved genomic tools are needed to further understand pathogenesis and drug resistance. We demonstrated the CRISPR-Cas9 system for use in P. falciparum by disrupting chromosomal loci and generating marker-free, single-nucleotide substitutions with high efficiency. Additionally, an artemisinin-resistant strain was generated by introducing a previously implicated polymorphism, thus illustrating the value of efficient genome editing in malaria research.

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Mesh：

Year:  2014        PMID： 24880488     DOI： 10.1038/nbt.2925

Source DB:  PubMed          Journal:  Nat Biotechnol        ISSN： 1087-0156            Impact factor:   54.908


  22 in total

Review 1.  CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation.

Authors:  Devaki Bhaya; Michelle Davison; Rodolphe Barrangou
Journal:  Annu Rev Genet       Date:  2011       Impact factor: 16.830

2.  Targeted gene disruption shows that knobs enable malaria-infected red cells to cytoadhere under physiological shear stress.

Authors:  B S Crabb; B M Cooke; J C Reeder; R F Waller; S R Caruana; K M Davern; M E Wickham; G V Brown; R L Coppel; A F Cowman
Journal:  Cell       Date:  1997-04-18       Impact factor: 41.582

3.  A molecular marker of artemisinin-resistant Plasmodium falciparum malaria.

Authors:  Frédéric Ariey; Benoit Witkowski; Chanaki Amaratunga; Johann Beghain; Anne-Claire Langlois; Nimol Khim; Saorin Kim; Valentine Duru; Christiane Bouchier; Laurence Ma; Pharath Lim; Rithea Leang; Socheat Duong; Sokunthea Sreng; Seila Suon; Char Meng Chuor; Denis Mey Bout; Sandie Ménard; William O Rogers; Blaise Genton; Thierry Fandeur; Olivo Miotto; Pascal Ringwald; Jacques Le Bras; Antoine Berry; Jean-Christophe Barale; Rick M Fairhurst; Françoise Benoit-Vical; Odile Mercereau-Puijalon; Didier Ménard
Journal:  Nature       Date:  2013-12-18       Impact factor: 49.962

4.  Negative selection of Plasmodium falciparum reveals targeted gene deletion by double crossover recombination.

Authors:  Manoj T Duraisingh; Tony Triglia; Alan F Cowman
Journal:  Int J Parasitol       Date:  2002-01       Impact factor: 3.981

5.  Yeast dihydroorotate dehydrogenase as a new selectable marker for Plasmodium falciparum transfection.

Authors:  Suresh M Ganesan; Joanne M Morrisey; Hangjun Ke; Heather J Painter; Kamal Laroiya; Margaret A Phillips; Pradipsinh K Rathod; Michael W Mather; Akhil B Vaidya
Journal:  Mol Biochem Parasitol       Date:  2011-01-18       Impact factor: 1.759

6.  Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes.

Authors:  Robert W Moon; Joanna Hall; Farania Rangkuti; Yung Shwen Ho; Neil Almond; Graham H Mitchell; Arnab Pain; Anthony A Holder; Michael J Blackman
Journal:  Proc Natl Acad Sci U S A       Date:  2012-12-24       Impact factor: 11.205

7.  Transfection of Plasmodium falciparum within human red blood cells.

Authors:  Y Wu; C D Sifri; H H Lei; X Z Su; T E Wellems
Journal:  Proc Natl Acad Sci U S A       Date:  1995-02-14       Impact factor: 11.205

8.  The role of N-terminus of Plasmodium falciparum ORC1 in telomeric localization and var gene silencing.

Authors:  Abhijit S Deshmukh; Sandeep Srivastava; Susann Herrmann; Ashish Gupta; Pallabi Mitra; Tim Wolf Gilberger; Suman Kumar Dhar
Journal:  Nucleic Acids Res       Date:  2012-02-29       Impact factor: 16.971

9.  Malaria parasites utilize both homologous recombination and alternative end joining pathways to maintain genome integrity.

Authors:  Laura A Kirkman; Elizabeth A Lawrence; Kirk W Deitsch
Journal:  Nucleic Acids Res       Date:  2013-10-01       Impact factor: 16.971

10.  Robust inducible Cre recombinase activity in the human malaria parasite Plasmodium falciparum enables efficient gene deletion within a single asexual erythrocytic growth cycle.

Authors:  Christine R Collins; Sujaan Das; Eleanor H Wong; Nicole Andenmatten; Robert Stallmach; Fiona Hackett; Jean-Paul Herman; Sylke Müller; Markus Meissner; Michael J Blackman
Journal:  Mol Microbiol       Date:  2013-03-26       Impact factor: 3.501
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  294 in total

Review 1.  Antimalarial drug resistance: linking Plasmodium falciparum parasite biology to the clinic.

Authors:  Benjamin Blasco; Didier Leroy; David A Fidock
Journal:  Nat Med       Date:  2017-08-04       Impact factor: 53.440

Review 2.  Understanding artemisinin-resistant malaria: what a difference a year makes.

Authors:  Rick M Fairhurst
Journal:  Curr Opin Infect Dis       Date:  2015-10       Impact factor: 4.915

3.  Reappraising the effects of artemisinin on the ATPase activity of PfATP6 and SERCA1a E255L expressed in Xenopus laevis oocytes.

Authors:  Stéphanie David-Bosne; Michael Voldsgaard Clausen; Hanne Poulsen; Jesper Vuust Møller; Poul Nissen; Marc le Maire
Journal:  Nat Struct Mol Biol       Date:  2016-01       Impact factor: 15.369

4.  Comparative Proteomics and Functional Analysis Reveal a Role of Plasmodium falciparum Osmiophilic Bodies in Malaria Parasite Transmission.

Authors:  Pablo Suárez-Cortés; Vikram Sharma; Lucia Bertuccini; Giulia Costa; Naa-Lamiley Bannerman; Anna Rosa Sannella; Kim Williamson; Michael Klemba; Elena A Levashina; Edwin Lasonder; Pietro Alano
Journal:  Mol Cell Proteomics       Date:  2016-07-18       Impact factor: 5.911

5.  Red Blood Cell Invasion by the Malaria Parasite Is Coordinated by the PfAP2-I Transcription Factor.

Authors:  Joana Mendonca Santos; Gabrielle Josling; Philipp Ross; Preeti Joshi; Lindsey Orchard; Tracey Campbell; Ariel Schieler; Ileana M Cristea; Manuel Llinás
Journal:  Cell Host Microbe       Date:  2017-06-14       Impact factor: 21.023

6.  The mitochondrial ribosomal protein L13 is critical for the structural and functional integrity of the mitochondrion in Plasmodium falciparum.

Authors:  Hangjun Ke; Swati Dass; Joanne M Morrisey; Michael W Mather; Akhil B Vaidya
Journal:  J Biol Chem       Date:  2018-04-06       Impact factor: 5.157

7.  Genetic mapping reveals that sinefungin resistance in Toxoplasma gondii is controlled by a putative amino acid transporter locus that can be used as a negative selectable marker.

Authors:  Michael S Behnke; Asis Khan; L David Sibley
Journal:  Eukaryot Cell       Date:  2014-12-05

8.  Flexible guide-RNA design for CRISPR applications using Protospacer Workbench.

Authors:  Cameron Ross MacPherson; Artur Scherf
Journal:  Nat Biotechnol       Date:  2015-06-29       Impact factor: 54.908

9.  Plasmodium falciparum translational machinery condones polyadenosine repeats.

Authors:  Slavica Pavlovic Djuranovic; Jessey Erath; Ryan J Andrews; Peter O Bayguinov; Joyce J Chung; Douglas L Chalker; James Aj Fitzpatrick; Walter N Moss; Pawel Szczesny; Sergej Djuranovic
Journal:  Elife       Date:  2020-05-29       Impact factor: 8.140

10.  Artemisinin resistance phenotypes and K13 inheritance in a Plasmodium falciparum cross and Aotus model.

Authors:  Juliana M Sá; Sarah R Kaslow; Michael A Krause; Viviana A Melendez-Muniz; Rebecca E Salzman; Whitney A Kite; Min Zhang; Roberto R Moraes Barros; Jianbing Mu; Paul K Han; J Patrick Mershon; Christine E Figan; Ramoncito L Caleon; Rifat S Rahman; Tyler J Gibson; Chanaki Amaratunga; Erika P Nishiguchi; Kimberly F Breglio; Theresa M Engels; Soundarapandian Velmurugan; Stacy Ricklefs; Judith Straimer; Nina F Gnädig; Bingbing Deng; Anna Liu; Ababacar Diouf; Kazutoyo Miura; Gregory S Tullo; Richard T Eastman; Sumana Chakravarty; Eric R James; Kenneth Udenze; Suzanne Li; Daniel E Sturdevant; Robert W Gwadz; Stephen F Porcella; Carole A Long; David A Fidock; Marvin L Thomas; Michael P Fay; B Kim Lee Sim; Stephen L Hoffman; John H Adams; Rick M Fairhurst; Xin-Zhuan Su; Thomas E Wellems
Journal:  Proc Natl Acad Sci U S A       Date:  2018-11-19       Impact factor: 11.205
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