| Literature DB >> 24859753 |
Gražvydas Lukinavičius1, Luc Reymond2, Elisa D'Este3, Anastasiya Masharina4, Fabian Göttfert3, Haisen Ta3, Angelika Güther5, Mathias Fournier6, Stefano Rizzo7, Herbert Waldmann7, Claudia Blaukopf8, Christoph Sommer8, Daniel W Gerlich8, Hans-Dieter Arndt5, Stefan W Hell3, Kai Johnsson9.
Abstract
We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.Entities:
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Year: 2014 PMID: 24859753 DOI: 10.1038/nmeth.2972
Source DB: PubMed Journal: Nat Methods ISSN: 1548-7091 Impact factor: 28.547