Literature DB >> 6863518

Pyrene actin: documentation of the validity of a sensitive assay for actin polymerization.

J A Cooper, S B Walker, T D Pollard.   

Abstract

The fluorescence of pyrene-labelled actin is much higher after polymerization. We have characterized in detail the polymerization properties of pyrene actin and report that native and pyrene actin are identical using the following criteria: (1) the time course of polymerization; (2) the elongation rate constants; (3) the intrinsic viscosity; and (4) the critical concentration. Native and pyrene actin copolymerize. Fluorescence of polymerized pyrene actin is 7-10 times higher than monomer. The fluorescent signal is proportional to polymer weight concentration and is insensitive to filament length distribution. Bleaching can be minimized by appropriate filters to allow continuous monitoring of signal. Measurements do not influence polymerization kinetics. This establishes that pyrene actin fluorescence is a valid assay for actin polymerization that is more sensitive than any other current assay.

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Year:  1983        PMID： 6863518     DOI： 10.1007/bf00712034

Source DB:  PubMed          Journal:  J Muscle Res Cell Motil        ISSN： 0142-4319            Impact factor:   2.698


  17 in total

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Authors:  A Wegner
Journal:  J Mol Biol       Date:  1976-11       Impact factor: 5.469

2.  The measurement of actin concentration in solution: a comparison of methods.

Authors:  T W Houk; K Ue
Journal:  Anal Biochem       Date:  1974-11       Impact factor: 3.365

3.  Conformational changes associated with polymerization and nucleotide binding in actin molecules.

Authors:  S Higashi; F Oosawa
Journal:  J Mol Biol       Date:  1965-07       Impact factor: 5.469

4.  Effect of the state of oxidation of cysteine 190 of tropomyosin on the assembly of the actin-tropomyosin complex.

Authors:  T P Walsh; A Wegner
Journal:  Biochim Biophys Acta       Date:  1980-11-20

5.  Regulation of actin polymerization by villin, a 95,000 dalton cytoskeletal component of intestinal brush borders.

Authors:  S W Craig; L D Powell
Journal:  Cell       Date:  1980-12       Impact factor: 41.582

6.  Methods to measure actin polymerization.

Authors:  J A Cooper; T D Pollard
Journal:  Methods Enzymol       Date:  1982       Impact factor: 1.600

7.  Fluorimetry study of N-(1-pyrenyl)iodoacetamide-labelled F-actin. Local structural change of actin protomer both on polymerization and on binding of heavy meromyosin.

Authors:  T Kouyama; K Mihashi
Journal:  Eur J Biochem       Date:  1981

8.  7-Chloro-4-nitrobenzeno-2-oxa-1,3-diazole actin as a probe for actin polymerization.

Authors:  P Detmers; A Weber; M Elzinga; R E Stephens
Journal:  J Biol Chem       Date:  1981-01-10       Impact factor: 5.157

9.  Mechanism of action of Acanthamoeba profilin: demonstration of actin species specificity and regulation by micromolar concentrations of MgCl2.

Authors:  P C Tseng; T D Pollard
Journal:  J Cell Biol       Date:  1982-07       Impact factor: 10.539

10.  Detection of actin assembly by fluorescence energy transfer.

Authors:  D L Taylor; J Reidler; J A Spudich; L Stryer
Journal:  J Cell Biol       Date:  1981-05       Impact factor: 10.539
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  193 in total

1.  Interaction of myosin with F-actin: time-dependent changes at the interface are not slow.

Authors:  J Van Dijk; F Céline; T Barman; P Chaussepied
Journal:  Biophys J       Date:  2000-06       Impact factor: 4.033

2.  Fesselin, a synaptopodin-like protein, stimulates actin nucleation and polymerization.

Authors:  B Beall; J M Chalovich
Journal:  Biochemistry       Date:  2001-11-27       Impact factor: 3.162

Review 3.  Actin-based motility of intracellular microbial pathogens.

Authors:  M B Goldberg
Journal:  Microbiol Mol Biol Rev       Date:  2001-12       Impact factor: 11.056

4.  Modulation of actin filament behavior by GAP-43 (neuromodulin) is dependent on the phosphorylation status of serine 41, the protein kinase C site.

Authors:  Q He; E W Dent; K F Meiri
Journal:  J Neurosci       Date:  1997-05-15       Impact factor: 6.167

5.  End versus side branching by Arp2/3 complex.

Authors:  A E Carlsson; M A Wear; J A Cooper
Journal:  Biophys J       Date:  2004-02       Impact factor: 4.033

Review 6.  Choosing an effective protein bioconjugation strategy.

Authors:  Nicholas Stephanopoulos; Matthew B Francis
Journal:  Nat Chem Biol       Date:  2011-11-15       Impact factor: 15.040

7.  A novel actin binding site of myosin required for effective muscle contraction.

Authors:  Boglárka H Várkuti; Zhenhui Yang; Bálint Kintses; Péter Erdélyi; Irén Bárdos-Nagy; Attila L Kovács; Péter Hári; Miklós Kellermayer; Tibor Vellai; András Málnási-Csizmadia
Journal:  Nat Struct Mol Biol       Date:  2012-02-12       Impact factor: 15.369

8.  An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability.

Authors:  Erin D Goley; Aravind Rammohan; Elizabeth A Znameroski; Elif Nur Firat-Karalar; David Sept; Matthew D Welch
Journal:  Proc Natl Acad Sci U S A       Date:  2010-04-19       Impact factor: 11.205

9.  Assembly of filopodia by the formin FRL2 (FMNL3).

Authors:  Elizabeth S Harris; Timothy J Gauvin; Ernest G Heimsath; Henry N Higgs
Journal:  Cytoskeleton (Hoboken)       Date:  2010-11-02

10.  Dimerized Drosophila myosin VIIa: a processive motor.

Authors:  Yi Yang; Mihály Kovács; Takeshi Sakamoto; Fang Zhang; Daniel P Kiehart; James R Sellers
Journal:  Proc Natl Acad Sci U S A       Date:  2006-04-03       Impact factor: 11.205
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