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Literature DB >> 24764280

An expanded set of fluorogenic sulfatase activity probes.

Elizabeth L Smith¹, Carolyn R Bertozzi, Kimberly E Beatty.

Abstract

Fluorogenic probes that are activated by an enzymatic transformation are ideally suited for profiling enzyme activities in biological systems. Here, we describe two fluorogenic enzyme probes, 3-O-methylfluorescein-sulfate and resorufin-sulfate, that can be used to detect sulfatases in mycobacterial lysates. Both probes were validated with a set of commercial sulfatases and used to reveal species-specific sulfatase banding patterns in a gel-resolved assay of mycobacterial lysates. The fluorogenic probes described here are suitable for various assays and provide a starting point for creating new sulfatase probes with improved selectivity for mycobacterial sulfatases.

Entities: CellLine Chemical Disease Gene Species

Keywords: Mycobacterium tuberculosis; fluorescent probes; hydrolases; protein imaging; sulfatases

Mesh：

Substances：

Year: 2014 PMID： 24764280 PMCID： PMC4084507 DOI： 10.1002/cbic.201400032

Source DB: PubMed Journal: Chembiochem ISSN： 1439-4227 Impact factor: 3.164

31 in total

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Review 5. Sulfate metabolism in mycobacteria.

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