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Literature DB >> 2315033

Direct sequencing of double-stranded DNA PCR products via removing the complementary strand with single-stranded DNA of an M13 clone.

S Gal¹, B Hohn.

Abstract

Mesh：

Substances：
DNA, Single-Stranded
DNA

Year: 1990 PMID： 2315033 PMCID： PMC330393 DOI： 10.1093/nar/18.4.1076

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

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5 in total

1. Sequence analysis of minute amounts of viroid RNA using the polymerase chain reaction (PCR). Rapid communication.

Authors: H Puchta; H L Sänger
Journal: Arch Virol Date: 1989 Impact factor: 2.574

2. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors: R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal: Science Date: 1988-01-29 Impact factor: 47.728

Review 3. PCR and DNA sequencing.

Authors: U B Gyllensten
Journal: Biotechniques Date: 1989 Jul-Aug Impact factor: 1.993

4. Direct sequencing from low-melt agarose with Sequenase.

Authors: K A Kretz; G S Carson; J S O'Brien
Journal: Nucleic Acids Res Date: 1989-07-25 Impact factor: 16.971

5. Characterization of beta-thalassaemia mutations using direct genomic sequencing of amplified single copy DNA.

Authors: C Wong; C E Dowling; R K Saiki; R G Higuchi; H A Erlich; H H Kazazian
Journal: Nature Date: 1987 Nov 26-Dec 2 Impact factor: 49.962

5 in total

1. A simple technique for generating probes for RNA in situ hybridization: an adjunct to genome mapping exemplified by the RAG-1/RAG-2 gene cluster.

Authors: T Boehm; R Gonzalez-Sarmiento; M Kennedy; T H Rabbitts
Journal: Proc Natl Acad Sci U S A Date: 1991-05-01 Impact factor: 11.205

Direct sequencing of double-stranded DNA PCR products via removing the complementary strand with single-stranded DNA of an M13 clone.

1. Sequence analysis of minute amounts of viroid RNA using the polymerase chain reaction (PCR). Rapid communication.

2. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Review 3. PCR and DNA sequencing.

4. Direct sequencing from low-melt agarose with Sequenase.

5. Characterization of beta-thalassaemia mutations using direct genomic sequencing of amplified single copy DNA.

1. A simple technique for generating probes for RNA in situ hybridization: an adjunct to genome mapping exemplified by the RAG-1/RAG-2 gene cluster.

2. Sequencing of double-stranded PCR products.

3. Thermal cycle dideoxy DNA sequencing.

Review 4. DNA sequencing. Recent innovations and future trends.

5. Genomic homologous recombination in planta.