Kelly Athayde Wirka1, Alice A Chen1, Joe Conaghan2, Kristen Ivani3, Marina Gvakharia4, Barry Behr5, Vaishali Suraj1, Lei Tan1, Shehua Shen6. 1. Auxogyn, Menlo Park, California. 2. Pacific Fertility Center, San Francisco, California. 3. Reproductive Science Center of the Bay Area, San Ramon, California. 4. Fertility Physicians of Northern California, Palo Alto Medical Foundation, San Jose, California. 5. Stanford Fertility and Reproductive Medicine Center, Palo Alto, California. 6. Auxogyn, Menlo Park, California. Electronic address: sshen@auxogyn.com.
Abstract
OBJECTIVE: To characterize atypical dynamic embryo phenotypes identified by time-lapse microscopy, evaluate their prevalence, and determine their association with embryo development. DESIGN: Retrospective multicenter cohort study. SETTING: Five IVF clinics in the United States. PATIENT(S): Sixty-seven women undergoing IVF treatment with 651 embryos. INTERVENTION(S): Embryo videos were retrospectively analyzed for atypical phenotypes. MAIN OUTCOME MEASURE(S): Identification of four groups of atypical embryo phenotypes: abnormal syngamy (AS), abnormal first cytokinesis (A1(cyt)), abnormal cleavage (AC), and chaotic cleavage (CC). Prevalence and association with embryo morphology and development potential were evaluated. RESULT(S): A high prevalence of atypical phenotypes was observed among embryos: AS 25.1% (163/649), A1(cyt) 31.0% (195/639), AC 18% (115/639) and CC 15% (96/639). A high percentage of embryos with atypical phenotype(s) had good quality on day 3 (overall grade good or fair): AS 78.6% (70/89); A1(cyt) 79.7% (94/119), AC 86.4% (70/81), and CC 35.2% (19/54), but the blastocyst formation rates for these embryos were significantly lower compared with their respective control groups: AS 21.5% vs. 44.9%, A1(cyt) 21.7% vs. 44.6%, AC 11.7% vs. 43.1%, and CC 14.0% vs. 42.3%. CONCLUSION(S): Embryos exhibiting atypical phenotypes are highly prevalent in human embryos and show significantly lower developmental potential than control embryos. CLINICAL TRIAL REGISTRATION NUMBER: NCT01369446.
OBJECTIVE: To characterize atypical dynamic embryo phenotypes identified by time-lapse microscopy, evaluate their prevalence, and determine their association with embryo development. DESIGN: Retrospective multicenter cohort study. SETTING: Five IVF clinics in the United States. PATIENT(S): Sixty-seven women undergoing IVF treatment with 651 embryos. INTERVENTION(S): Embryo videos were retrospectively analyzed for atypical phenotypes. MAIN OUTCOME MEASURE(S): Identification of four groups of atypical embryo phenotypes: abnormal syngamy (AS), abnormal first cytokinesis (A1(cyt)), abnormal cleavage (AC), and chaotic cleavage (CC). Prevalence and association with embryo morphology and development potential were evaluated. RESULT(S): A high prevalence of atypical phenotypes was observed among embryos: AS 25.1% (163/649), A1(cyt) 31.0% (195/639), AC 18% (115/639) and CC 15% (96/639). A high percentage of embryos with atypical phenotype(s) had good quality on day 3 (overall grade good or fair): AS 78.6% (70/89); A1(cyt) 79.7% (94/119), AC 86.4% (70/81), and CC 35.2% (19/54), but the blastocyst formation rates for these embryos were significantly lower compared with their respective control groups: AS 21.5% vs. 44.9%, A1(cyt) 21.7% vs. 44.6%, AC 11.7% vs. 43.1%, and CC 14.0% vs. 42.3%. CONCLUSION(S): Embryos exhibiting atypical phenotypes are highly prevalent in human embryos and show significantly lower developmental potential than control embryos. CLINICAL TRIAL REGISTRATION NUMBER: NCT01369446.
Authors: N L Sandi-Monroy; S Musanovic; D Zhu; K Eibner; N Reeka; J Koglin; K Bundschu; F Gagsteiger Journal: J Assist Reprod Genet Date: 2020-02-06 Impact factor: 3.412
Authors: Rajiv C McCoy; Louise J Newnham; Christian S Ottolini; Eva R Hoffmann; Katerina Chatzimeletiou; Omar E Cornejo; Qiansheng Zhan; Nikica Zaninovic; Zev Rosenwaks; Dmitri A Petrov; Zachary P Demko; Styrmir Sigurjonsson; Alan H Handyside Journal: Hum Mol Genet Date: 2018-07-15 Impact factor: 6.150
Authors: Matthew D VerMilyea; Lei Tan; Joshua T Anthony; Joe Conaghan; Kristen Ivani; Marina Gvakharia; Robert Boostanfar; Valerie L Baker; Vaishali Suraj; Alice A Chen; Monica Mainigi; Christos Coutifaris; Shehua Shen Journal: Reprod Biomed Online Date: 2014-09-21 Impact factor: 3.828