Minyue Ma1, Shihui Zhang1, Chongzhao Lu1, Shuling Wang1, Yuanqing Yao1, Hongmei Peng2. 1. Reproductive Medicine Center, Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing, 100853, China. 2. Reproductive Medicine Center, Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing, 100853, China. phmeizys@hotmail.com.
Abstract
PURPOSE: To study the chromosome constitution of equal-sized three-cell embryo. METHODS: We determined the chromosome constitution of 105 blastomeres from 35 embryos using multiple annealing and looping-based amplification cycles (MALBAC) together with NGS sequencing technology. Chromosomal copy number variation (CNV) analysis was successfully performed in 27 embryos. We also analyzed radius, perimeter, area, and volume of each blastomere to explore the possibility of selecting the normal embryos. RESULTS: Majority of the embryos (77.8%, 21/27) studied were mosaic or aneuploid, and only 22.2% (6/27) had normal chromosome numbers. The aneuploid chromosomes spread across all chromosomes and the most frequent aneuploidies were for chromosomes 1, 16, and 18 followed by 13, 19, and 21. Statistical analyses showed no significant difference between euploid and aneuploid embryos regarding radius, perimeter, area, and volume of their blastomeres. CONCLUSIONS: Our results showed that majority of the equal-sized three-cell embryos were chromosomally abnormal and could not be distinguished by morphology observation, so they should be given lower priority at selection for transfer.
PURPOSE: To study the chromosome constitution of equal-sized three-cell embryo. METHODS: We determined the chromosome constitution of 105 blastomeres from 35 embryos using multiple annealing and looping-based amplification cycles (MALBAC) together with NGS sequencing technology. Chromosomal copy number variation (CNV) analysis was successfully performed in 27 embryos. We also analyzed radius, perimeter, area, and volume of each blastomere to explore the possibility of selecting the normal embryos. RESULTS: Majority of the embryos (77.8%, 21/27) studied were mosaic or aneuploid, and only 22.2% (6/27) had normal chromosome numbers. The aneuploid chromosomes spread across all chromosomes and the most frequent aneuploidies were for chromosomes 1, 16, and 18 followed by 13, 19, and 21. Statistical analyses showed no significant difference between euploid and aneuploid embryos regarding radius, perimeter, area, and volume of their blastomeres. CONCLUSIONS: Our results showed that majority of the equal-sized three-cell embryos were chromosomally abnormal and could not be distinguished by morphology observation, so they should be given lower priority at selection for transfer.
Authors: A H Sathananthan; J J Tarin; L Gianaroli; S C Ng; V Dharmawardena; M C Magli; R Fernando; A O Trounson Journal: Hum Reprod Update Date: 1999 Sep-Oct Impact factor: 15.610
Authors: E B Baart; E Martini; I van den Berg; N S Macklon; R-J H Galjaard; B C J M Fauser; D Van Opstal Journal: Hum Reprod Date: 2005-09-09 Impact factor: 6.918
Authors: D Hlinka; B Kaľatová; I Uhrinová; S Dolinská; J Rutarová; J Rezáčová; S Lazarovská; M Dudáš Journal: Physiol Res Date: 2012-08-08 Impact factor: 1.881
Authors: David Gisselsson; Yuesheng Jin; David Lindgren; Johan Persson; Lennart Gisselsson; Sandra Hanks; Daniel Sehic; Linda Holmquist Mengelbier; Ingrid Øra; Nazneen Rahman; Fredrik Mertens; Felix Mitelman; Nils Mandahl Journal: Proc Natl Acad Sci U S A Date: 2010-11-08 Impact factor: 11.205