Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Structure of the yeast mitochondrial large ribosomal subunit.

Literature DB >> 24675956

Structure of the yeast mitochondrial large ribosomal subunit.

Alexey Amunts¹, Alan Brown¹, Xiao-Chen Bai¹, Jose L Llácer¹, Tanweer Hussain¹, Paul Emsley¹, Fei Long¹, Garib Murshudov¹, Sjors H W Scheres¹, V Ramakrishnan¹.

Abstract

Mitochondria have specialized ribosomes that have diverged from their bacterial and cytoplasmic counterparts. We have solved the structure of the yeast mitoribosomal large subunit using single-particle cryo-electron microscopy. The resolution of 3.2 angstroms enabled a nearly complete atomic model to be built de novo and refined, including 39 proteins, 13 of which are unique to mitochondria, as well as expansion segments of mitoribosomal RNA. The structure reveals a new exit tunnel path and architecture, unique elements of the E site, and a putative membrane docking site.

Entities: Chemical Disease Gene Mutation Species

Mesh：

Substances：

Year: 2014 PMID： 24675956 PMCID： PMC4046073 DOI： 10.1126/science.1249410

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

37 in total

1. The complete atomic structure of the large ribosomal subunit at 2.4 A resolution.

Authors: N Ban; P Nissen; J Hansen; P B Moore; T A Steitz
Journal: Science Date: 2000-08-11 Impact factor: 47.728

2. Cell biology. Irremediable complexity?

Authors: Michael W Gray; Julius Lukes; John M Archibald; Patrick J Keeling; W Ford Doolittle
Journal: Science Date: 2010-11-12 Impact factor: 47.728

3. A structural model for the large subunit of the mammalian mitochondrial ribosome.

Authors: Jason A Mears; Manjuli R Sharma; Robin R Gutell; Amanda S McCook; Paul E Richardson; Thomas R Caulfield; Rajendra K Agrawal; Stephen C Harvey
Journal: J Mol Biol Date: 2006-02-10 Impact factor: 5.469

4. Mrpl36 is important for generation of assembly competent proteins during mitochondrial translation.

Authors: Martin Prestele; Frank Vogel; Andreas S Reichert; Johannes M Herrmann; Martin Ott
Journal: Mol Biol Cell Date: 2009-04-01 Impact factor: 4.138

Review 5. Co-translational membrane insertion of mitochondrially encoded proteins.

Authors: Martin Ott; Johannes M Herrmann
Journal: Biochim Biophys Acta Date: 2009-12-02

6. Features and development of Coot.

Authors: P Emsley; B Lohkamp; W G Scott; K Cowtan
Journal: Acta Crystallogr D Biol Crystallogr Date: 2010-03-24

7. Antibiotic susceptibility of the peptidyl transferase locus of bovine mitochondrial ribosomes.

Authors: N D Denslow; T W O'Brien
Journal: Eur J Biochem Date: 1978-11-15

Review 8. Yeast models of human mitochondrial diseases.

Authors: Antoni Barrientos
Journal: IUBMB Life Date: 2003-02 Impact factor: 3.885

9. Cbp3-Cbp6 interacts with the yeast mitochondrial ribosomal tunnel exit and promotes cytochrome b synthesis and assembly.

Authors: Steffi Gruschke; Kirsten Kehrein; Katharina Römpler; Kerstin Gröne; Lars Israel; Axel Imhof; Johannes M Herrmann; Martin Ott
Journal: J Cell Biol Date: 2011-06-13 Impact factor: 10.539

10. RELION: implementation of a Bayesian approach to cryo-EM structure determination.

Authors: Sjors H W Scheres
Journal: J Struct Biol Date: 2012-09-19 Impact factor: 2.867

255 in total

Review 1. Two promising future developments of cryo-EM: capturing short-lived states and mapping a continuum of states of a macromolecule.

Authors: Bo Chen; Joachim Frank
Journal: Microscopy (Oxf) Date: 2015-10-31 Impact factor: 1.571

Structure of the yeast mitochondrial large ribosomal subunit.

1. The complete atomic structure of the large ribosomal subunit at 2.4 A resolution.

2. Cell biology. Irremediable complexity?

3. A structural model for the large subunit of the mammalian mitochondrial ribosome.

4. Mrpl36 is important for generation of assembly competent proteins during mitochondrial translation.

Review 5. Co-translational membrane insertion of mitochondrially encoded proteins.

6. Features and development of Coot.

7. Antibiotic susceptibility of the peptidyl transferase locus of bovine mitochondrial ribosomes.

Review 8. Yeast models of human mitochondrial diseases.

9. Cbp3-Cbp6 interacts with the yeast mitochondrial ribosomal tunnel exit and promotes cytochrome b synthesis and assembly.

10. RELION: implementation of a Bayesian approach to cryo-EM structure determination.

Review 1. Two promising future developments of cryo-EM: capturing short-lived states and mapping a continuum of states of a macromolecule.

Review 2. Generalized single-particle cryo-EM--a historical perspective.

3. Structure of the eukaryotic MCM complex at 3.8 Å.

Review 4. Single-particle cryo-electron microscopy of macromolecular complexes.

5. De Novo modeling in cryo-EM density maps with Pathwalking.

6. Cryo-EM structures of apo and antagonist-bound human Ca_v3.1.

7. Evaluation of super-resolution performance of the K2 electron-counting camera using 2D crystals of aquaporin-0.

8. Ribosomes are optimized for autocatalytic production.

9. The yeast protein Mam33 functions in the assembly of the mitochondrial ribosome.

Review 10. Single-Particle Cryo-EM at Crystallographic Resolution.